摘要
bHLH转录因子家族不仅参与了植物的生长发育,而且在植物响应逆境胁迫和次生代谢方面发挥着关键作用。在全基因组水平对重要饲草及能源植物象草的bHLH转录因子家族进行了鉴定及分析,并利用转录组数据及定量RT-PCR分析了象草bHLH转录因子对赤霉素(GA_(3))和多效唑(PAC)的响应。结果显示:在象草中共鉴定出229个具有完整保守结构域的bHLH基因家族成员(CpbHLH001~CpbHLH229),不均匀地分布于14条染色体上;系统进化分析结果表明,229个CpbHLHs可被分为18个亚类,其中C亚类的成员数量最多,为41个;此外,相同亚家族中的大多数基因具有相似的基因结构和保守基序;基于转录组数据的表达谱分析结果发现,多数bHLH基因在象草茎尖组织中均对GA_(3)和PAC有响应。随机挑选9个表达量较高的基因进一步通过qPCR进行验证。结果显示,经外源GA_(3)和PAC处理之后,这9个基因因不同处理而差异表达,表明这9个基因可能与GA_(3)和PAC介导的信号通路有关。综上所述,本研究为象草bHLH转录因子家族的生物学功能奠定了基础。
The bHLH(basic helix-loop-helix)transcription factor family not only participates in plant growth and development,but also plays a key role in plant response to stress and secondary metabolism.In this study,we identified and analyzed at the whole genome level,the bHLH transcription factor family of Cenchrus purpureus(also called elephant grass,an important forage grass and energy plant).We also analyzed the response of bHLH transcription factors to gibberellin(GA_(3)) and paclobutrazol(PAC) by using transcriptome data and quantitative RT-PCR.A total of 229 bHLH gene family members(CpbHLH001-CpbHLH229),with complete conserved domains,and unevenly distributed on the 14 chromosomes,were identified.The results of phylogenetic analysis revealed that 229 CpbHLHs could be classified into 18 subgroups,of which a subgroup designated C contained the highest number(41).The members of each subfamily had similar gene structures and conserved motifs.Expression profile analysis based on transcriptome data found that most bHLH genes responded to GA_(3) and PAC in the meristems of the shoot apex,and nine genes with higher expression were randomly selected and further verified by qRT-PCR.It was found that these nine genes were differentially expressed in response to different exogenous GA_(3) and PAC treatments,suggesting that these genes are associated with GA_(3) and PAC mediated signaling pathways.This study lays the foundation for further research on the biological functions of the bHLH transcription factor family in elephant grass.
作者
高莉娟
张正社
文裕
宗西方
闫启
卢丽燕
易显凤
张吉宇
GAO Li-juan;ZHANG Zheng-she;WEN Yu;ZONG Xi-fang;YAN Qi;LU Li-yan;YI Xian-feng;ZHANG Ji-yu(State Key Laboratory of Grassland Agroecosystems,Key Laboratory of Grassland Livestock Industry Innovation,Ministry of Agriculture and Rural Affairs,College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,China;Guangxi Agricultural Vocational College,Nanning 530007,China;Guangxi Institute of Animal Sciences,Nanning 530001,China)
出处
《草业学报》
CSCD
北大核心
2022年第3期47-59,共13页
Acta Prataculturae Sinica
基金
广西壮族自治区科学技术厅项目(桂科AD17129043,桂科AB19245024)资助。
关键词
象草
bHLH转录因子
基因鉴定
表达分析
elephant grass
bHLH transcription factor
gene identification
gene expression pattern
