摘要
环境胁迫严重影响花生的产量和品质。筛选并研究花生中胁迫响应相关基因,对深入解析花生响应胁迫的分子机理具有重要意义。本研究从花生果腐病转录组文库中筛选并克隆得到花生基因AhMAPK14,对其进行了生物信息学分析及亚细胞定位,并利用qRT-PCR技术分析了其组织表达特异性及在不同处理下的表达模式。结果表明,AhMAPK14具有MAPK家族的典型结构域,并且在多数植物中高度保守;定位于细胞质与细胞核中,与多数MAPK家族成员的定位一致。因此推测AhMAPK14在花生中发挥的作用与其他植物相类似。此外,AhMAPK14在不同组织中的表达量差异性较大,在下胚轴中的相对表达量最高,并且能响应干旱、低温等非生物胁迫和ABA、JA等多种植物激素及信号分子的刺激。以上结果初步表明AhMAPK14在花生应对逆境胁迫中发挥作用,为深入揭示AhMAPK14的功能和花生抵御环境胁迫的分子机理打下了基础。
Environmental stress seriously affects the yield and quality of peanut. Screening and studying genes related to stress response in peanut is of great significance for revealing the molecular mechanism of peanut response to stress. The peanut AhMAPK14 gene was screened and cloned in this experiment, and its bioinformatics analysis and subcellular localization were conducted. The expression specificity of AhMAPK14 in tissues was analyzed and the expression patterns of AhMAPK14 under different treatments were detected by qRT-PCR. The results showed that AhMAPK14 has a typical domain of MAPK family and is highly conserved in most plants. It is located in cytoplasm and nucleus, and is consistent with most MAPK family members. Therefore, it is speculated that AhMAPK14 plays a similar role in peanut as in other plants. In addition, the expression level of AhMAPK14 varies greatly among different tissues, and the relative expression level of AhMAPK14 is the highest in hypocotyl, and it can respond to abiotic stress such as drought and low temperature, as well as stimulation from various plant hormones and signaling molecules such as ABA and JA. These results preliminarily indicated that AhMAPK14 plays a role in peanut response to environmental stress, which laid a foundation for further identifying on its function and studying the molecular mechanism of peanut resistance to environmental stress.
作者
朱立飞
张初署
唐月异
陈娜
潘丽娟
迟晓元
张建成
王通
王冕
ZHU Li-fei;ZHANG Chu-shu;TANG Yue-yi;CHEN Na;PAN Li-juan;CHI Xiao-yuan;ZHANG Jian-cheng;WANG Tong;WANG Mian(Shandong Peanut Research Institute/Key Lab.of Biology,Genetics and Breeding of Peanut,Ministry of Agriculture and Rural Affairs/Shandong Key Lab.of Peanut,Qingdao 266100,China)
出处
《花生学报》
北大核心
2022年第1期1-8,16,共9页
Journal of Peanut Science
基金
山东省自然基金面上项目(ZR2020MC103)
山东省农科院科技创新工程(CXGC2021C07)
青岛市民生计划项目(19-6-1-61-nsh)。
