摘要
目的探讨血清miR-296-5p及胃蛋白酶原mRNA表达水平对胃癌诊断和预后评估的临床价值。方法选取该院2014年3月至2019年3月收治且经病理证实的胃癌患者198例为胃癌组,198例胃良性疾病患者为胃病组,198例体检健康者为对照组,采用RT-qPCR法检测血清中miR-296-5p、胃蛋白酶原mRNA表达水平。分析不同组及不同特征胃癌患者血清miR-296-5p及胃蛋白酶原mRNA表达水平差异,采用受试者工作特征曲线(ROC曲线)分析血清miR-296-5p及胃蛋白酶原mRNA对胃癌的诊断价值。结果胃癌组和胃病组miR-296-5p、胃蛋白酶原mRNA表达水平高于对照组(P<0.05);胃癌组miR-296-5p、胃蛋白酶原mRNA表达水平高于胃病组(P<0.05)。miR-296-5p、胃蛋白酶原mRNA表达水平与胃癌患者性别、年龄无关(P>0.05);与肿瘤最大径、TNM分期、浸润深度、淋巴结转移和复发有关,且肿瘤最大径≥5 cm、TNM分期越高、浸润深度越深、有淋巴结转移、有复发,miR-296-5p、胃蛋白酶原mRNA高表达率越高(P<0.05)。miR-296-5p、胃蛋白酶原mRNA高表达组总生存率及生存期明显低于miR-296-5p、胃蛋白酶原mRNA低表达组(P<0.05)。miR-296-5p、胃蛋白酶原mRNA联合检测诊断胃癌的灵敏度、特异度、准确度、曲线下面积(AUC)均高于miR-296-5p、胃蛋白酶原mRNA单独检测(P<0.05)。结论胃癌患者血清miR-296-5p及胃蛋白酶原mRNA表达水平升高,miR-296-5p、胃蛋白酶原mRNA与胃癌患者临床病理特征、预后有关;miR-296-5p、胃蛋白酶原mRNA联合检测诊断胃癌具有较高的灵敏度、特异度,可在临床上应用。
Objective To investigate the clinical value of serum microRNA-296-5p(miR-296-5p)and pepsinogen mRNA expression level in the diagnosis and prognosis evaluation of gastric cancer.Methods A total of 198 patients with gastric cancer confirmed by pathology in our hospital from March 2014 to March 2019 were selected as the gastric cancer group,198 patients with benign gastric diseases were selected as the gastric disease group,and 198 healthy subjects were selected as the control group.The RT-qPCR method was used to detect serum miR-296-5p and proteasome mRNA expression level.The differences in serum miR-296-5p and pepsinogen mRNA levels in different groups and patients with different characteristics of gastric cancer were analyzed,and ROC curve was used to analyze the diagnostic value of serum miR-296-5p and pepsinogen mRNA in gastric cancer.Results The expression levels of miR-296-5p and pepsinogen mRNA in the gastric cancer group and gastric disease group were higher than those in the control group(P<0.05),and the expression levels of miR-296-5p and pepsinogen mRNA in the gastric cancer group were higher than those in the gastric disease group(P<0.05).The expression of miR-296-5p and pepsinogen mRNA were not significantly correlated with the gender and age of gastric cancer patients(P>0.05),and they were significantly correlated with the largest tumor diameter,TNM stage,depth of invasion,lymph node metastasis,recurrence,and the largest tumor diameter≥5 cm,the higher the TNM stage,the deeper the depth of invasion,lymph node metastasis,and recurrence,the higher the positive expression rates of miR-296-5p and pepsinogen mRNA(P<0.05).The AUC,sensitivity,specificity and accuracy of miR-296-5p combined with pepsinogen mRNA in diagnosing gastric cancer were significantly higher than miR-296-5p and pepsinogen mRNA detecting alone(P<0.05).Conclusion Serum miR-296-5p and pepsinogen mRNA are elevated in patients with gastric cancer.MiR-296-5p and pepsinogen mRNA are related to the clinicopathological characteristics and prognosis of patients with gastric cancer.Detection of miR-296-5p combined with pepsinogen mRNA in diagnosing gastric cancer has a higher rate of sensitivity and specificity screening,which can be used in clinical practice.
作者
白菊芳
许国彩
刘芝兰
薛晓红
BAI Jufang;XU Guocai;LIU Zhilan;XUE Xiaohong(Department of Gastroenterology,Qinghai Provincial People′s Hospital,Xining,Qinghai 810000,China)
出处
《国际检验医学杂志》
CAS
2022年第6期754-758,共5页
International Journal of Laboratory Medicine
