摘要
考虑到微生物污染引起的相关安全性问题,为了改善壳聚糖材料的抗菌性能,提高壳聚糖基抗菌材料的进一步应用,本文利用等离子体技术,在氦气氛围下对壳聚糖材料进行改性处理,设计得到改性壳聚糖抗菌材料,并通过抗菌实验、游离蛋白/核酸等的测定,探讨改性壳聚糖材料的抗菌性能与作用机制。通过改性壳聚糖结构分析,壳聚糖材料经氦气等离子体改性后,光斑区和非光斑区表面均变得粗糙,但光斑区的粗糙程度更加明显,材料表面的-NH2比例下降(83.32%下降到72.10%),而-CONH2的比例上升(16.68%上升到27.90%),这与改性过程中带电粒子的碰撞及壳聚糖材料中化学键的断裂有关。抗菌实验结果表明,相比于未改性材料,等离子体改性壳聚糖材料显著提高大肠杆菌的抗菌性能(P<0.05),而对于金黄色葡萄球菌,改性前后并没有显著性差异(P>0.05)。改性壳聚糖材料表面的化学基团变化,其与细菌接触时,能明显改变细菌细胞膜通透性,并局部形成不利于细菌生长的环境,从而达到抗菌的效果。
Considering the relevant safety problems caused by microbial pollution,in order to improve the antibacterial properties of chitosan materials and its further application,this study modified chitosan(CS)materials with plasma under helium atmosphere to design CS-based antibacterial materials.In order to discuss the antibacterial properties and action mechanism of modified CS materials,which through antibacterial experiments,free protein/nucleic acid determination and so on.The microstructure of modified CS materials were characterized,and the results showed that after the CS materials were modified by helium plasma,the surface became rough,and the roughness was more obvious,The results also showed the-NH2 of CS-based materials decreased from 83.32%to 72.10%and the-CONH2 increased from 16.68%to 27.90%,related to the collision of charged particles during plasma modification.Moreover,in the antibacterial experiment,these CSbased materials showed excellent antibacterial properties to Escherichia coli(P<0.05)but for Staphylococcus aureus,there was no significant difference before and after modification(P>0.05).With the changes of chemical linkages,the cell membrane permeability of bacterium was significantly changed when contacted with the CS-based materials.There was also locally formed a negative environment to bacterium growth,which contributed to the antibacterial effect of materials.
作者
许俊聪
黎耀元
徐彩玲
司徒文贝
XU Juncong;LI Yaoyuan;XU Cailing;SITU Wenbei(College of Food Science,South China Agricultural University,Guangzhou 510640,China)
出处
《食品工业科技》
CAS
北大核心
2022年第7期110-118,共9页
Science and Technology of Food Industry
基金
国家自然科学基金资助项目(31601422)
广州市科技计划项目珠江新星专题(201806010184)。
关键词
等离子体改性
壳聚糖材料
微观结构
抗菌性能
结构分析
plasma modified
chitosan materials
microstructure
antibacterial properties
structure analysis
