摘要
红肉的大量摄入会导致其未消化成分经肠道菌群发酵形成有害代谢产物,从而破坏肠稳态,增加患结肠癌、心血管疾病的风险。为探究添加菊粉和大豆膳食纤维对牛肉饮食小鼠的肠道菌群及代谢产物的影响,本实验将雄性C57BL/6Cnc小鼠随机分为空白对照组、牛肉组、牛肉与菊粉组和牛肉与大豆膳食纤维组,通过16S rRNA高通量测序技术检测分析小鼠肠道菌群结构,使用气相色谱质谱联用法检测结肠内容物的短链脂肪酸(short-chain fatty acids,SCFAs)含量,并测定小鼠肾脏氧化三甲胺(trimetlylamine oxide,TMAO)质量浓度。结果显示:牛肉组小鼠肠道菌群多样性显著降低(P<0.05),厚壁菌门、乳杆菌属和狭义梭菌属丰度增加,双歧杆菌和粪杆菌属等有益菌丰度减少,结肠内SCFAs含量下降,体内TMAO质量浓度显著升高(P<0.05);菊粉和大豆膳食纤维的添加均能显著提高小鼠肠道菌群多样性(P<0.05),菊粉可增加双歧杆菌和Ruminococcaceae_UCG_014丰度,大豆膳食纤维升高了阿克曼氏菌属和粪杆菌属丰度。两种膳食纤维均显著增加了结肠内SCFAs含量,显著降低了体内TMAO质量浓度(P<0.05)。综上,牛肉摄入确实会对小鼠肠道菌群及其代谢产物产生负面影响,而两种膳食纤维的添加可以在一定程度上改善牛肉饮食的不良影响,从而降低多种相关疾病的罹患风险。该研究结果可为合理膳食及未来个性化精准摄取营养提供理论科学依据。
Undigested components from a large intake of red meat can be fermented by the intestinal flora to form harmful metabolites,thereby destroying intestinal homeostasis and increasing the risk of colon cancer and cardiovascular diseases.To explore the effect of dietary addition of inulin or soybean dietary fiber(SDF)on the intestinal flora and metabolites in mice fed a beef-containing diet,male C57BL/6Cnc mice were randomly divided into a blank control group,a beef group,a beef plus inulin group,and a beef plus SDF group.The intestinal flora structure of mice was detected by high-throughput 16S rRNA gene sequencing.The concentrations of short-chain fatty acids(SCFAs)in the colon contents were detected by gas chromatography-mass spectrometry(GC-MS),and the content of trimethylamine oxide(TMAO)in the kidney was determined.The results showed that in the beef group,the diversity of intestinal flora decreased significantly(P<0.05),the abundance of Firmicutes,Lactobacillus and Clostridium_sensu_stricto_1 increased,and the abundance of beneficial bacteria such as Bifidobacterium and Faecalibaculum decreased;the contents of SCFAs in the colon contents decreased,and the content of TMAO increased significantly(P<0.05).Dietary addition of inulin or SDF significantly improved the diversity of intestinal flora in mice(P<0.05);inulin increased the abundance of Bifidobacterium and Ruminococcaceae_UCG_014,while SDF increased the abundance of Akkermansia and Faecalibaculum.Both dietary fibers increased the content of SCFAs in the colon contents and decreased the concentration of TMAO in the kidney(P<0.05).In conclusion,beef intake does have a negative impact on the intestinal flora and metabolites in mice,and this effect can be blocked by addition of dietary fiber to the diet,thereby reducing the risk of various related diseases.These results can provide a theoretical and scientific basis for the development of a reasonable diet and personalized precise nutrition in the future.
作者
武明月
孔祥丽
张天阳
冯熙瑞
徐境含
许晓曦
WU Mingyue;KONG Xiangli;ZHANG Tianyang;FENG Xirui;XU Jinghan;XU Xiaoxi(Key Laboratory of Dairy Science,Ministry of Education,Northeast Agricultural University,Harbin 150030,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2022年第5期158-167,共10页
Food Science
关键词
牛肉
菊粉
大豆膳食纤维
肠道菌群
短链脂肪酸
氧化三甲胺
beef
inulin
soybean dietary fiber
intestinal flora
short-chain fatty acid
trimethylamine oxide
