摘要
肉类掺假现象严重威胁公共卫生安全。快速、准确、可靠的动物源性成分检测技术是有效监管与检测肉类掺假的关键。总结了常见的掺假形式,并对物理技术、光谱技术、免疫学技术、DNA分析等检测方法进行了全面的总结归纳和比较分析,尤其是对当前热门的DNA分析方法,详细阐述了常规PCR、实时荧光定量PCR、数字PCR和等温扩增PCR这4种主要分子检测技术的优缺点和应用情况,旨在为未来动物源性检测技术的发展指明方向。
Meat adulteration has seriously threatened public health security.Fast,accurate and reliable animal-derived compo‑nent detection technology is the key to effective supervision and detection of meat adulteration.This article summarized the com‑mon forms of adulteration,and comprehensively summarized and analyzed various methods such as physical,spectroscopy,im‑munology,and DNA analysis.Especially for the current popular DNA methods,it elaborated four main molecular detection tech‑nologies of conventional PCR,real-time fluorescent quantitative PCR,digital PCR and isothermal amplification PCR,and the advantages,disadvantages and applications of these technologies were discussed.This article was expected to point out the future development direction of animal-derived component detection technology.
作者
余卉茹
纪艺
彭城
徐晓丽
汪小福
孙梅好
陈笑芸
YU Huiru;JI Yi;PENG Cheng;XU Xiaoli;WANG Xiaofu;SUN Meihao;CHEN Xiaoyun(School of Chemistry and Life Sciences,Zhejiang Normal University,Zhejiang Jinhua 321000,China;Institute of Agro‑Product Safety and Nutrition,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)
出处
《生物技术进展》
2022年第2期213-221,共9页
Current Biotechnology
基金
浙江省科技计划项目(2021C02059)。
关键词
肉类掺假
动物源性成分检测技术
DNA分析方法
meat adulteration
animal-derived component detection technology
DNA analytical method
