摘要
目的探讨PTENP1对结直肠癌细胞增殖和凋亡的影响及其分子机制。方法选取107例结直肠癌及对应的癌旁组织作为研究对象,采用荧光定量PCR法检测结肠癌及癌旁组织中PTENP1表达水平。采用慢病毒在结肠癌HT29细胞建立PTENP1过表达细胞系(PTENP1组)和空载体细胞系(对照组)。CCK8试剂盒分析细胞的增殖能力,流式细胞术分析细胞的凋亡水平,生物信息学和双荧光素酶报告基因分析PTENP1靶基因,Western blot法检测细胞靶蛋白表达水平。结果与癌旁组织比较,结直肠癌组织中PTENP1表达水平显著下调(P<0.05)。对照组PTENP1表达水平明显低于PTENP1组(P<0.05)。与对照组比较,PTENP1组细胞增殖能力明显下降(P<0.05),细胞凋亡水平明显增加(P<0.05)。miR-21与PTENP1碱基互补。与对照组比较,PTENP1组细胞中miR-21表达水平显著下调(P<0.05)。PTEN蛋白表达水平显著上调(P<0.05)。结论PTENP1与miR-21竞争性结合,通过调节下游靶蛋白PTEN表达水平,进而影响结直肠癌细胞的增殖和凋亡。
Objective To investigate the effect of PTENP1 on the proliferation and apoptosis of colorectal cancer cells and its molecular mechanism.Methods We selected 107 cases of colorectal cancer and corresponding adjacent tissues as the research objects.The expression level of PTENP1 was analyzed by fluorescence quantitative PCR.Colon cancer HT29 cells with PTENP1 overexpression(PTENP1 group)and empty vector cell line(control group)were established by lentivirus.The cell proliferation and apoptosis were analyzed by CCK8 and flow cytometry.The PTENP1 target gene was analyzed by bioinformatics and double luciferase reporter genes.The expression level of target protein was analyzed by Western blot.Results The expression of PTENP1 in colorectal cancer tissues was significantly lower than that in adjacent tissues(P<0.05).The expression level of PTENP1 in the control group was significantly lower than that in the PTENP1 group(P<0.05).Compared with the control group,the cell proliferation ability of the PTENP1 group was significantly decreased(P<0.05),the apoptosis level was significantly increased(P<0.05).miR-21 was complementary to PTENP1.Compared with the control group,the expression of miR-21 in the PTENP1 group was significantly down-regulated(P<0.05),and the expression of PTEN protein was significantly upregulated(P<0.05).Conclusion PTENP1 and miR-21 competitively bind to regulate the expression of PTEN,and then affect the proliferation and apoptosis of colorectal cancer cells.
作者
胡晓舒
温一阳
杨金花
HU Xiaoshu;WEN Yiyang;YANG Jinhua(Department of Pathology,People’s Hospital of Zhengzhou,Zhengzhou 450003,China;Department of Oncology,Henan Provincial People’s Hospital,People’s Hospital of Zhengzhou University,People’s Hospital of Henan University,Zhengzhou 450003,China)
出处
《肿瘤防治研究》
CAS
CSCD
2022年第3期192-196,共5页
Cancer Research on Prevention and Treatment
基金
河南省医学科技攻关计划(201602244)。
