摘要
为研究烟管头草(Carpesium cernuum)的化学成分及其细胞毒活性,采用多种色谱分离技术从烟管头草的95%乙醇提取物的乙酸乙酯萃取物中分离得到10个化合物。通过现代波谱技术鉴定了它们的结构,分别为(5 Z)-6-[(acetyloxy)methyl]-2-oxo-2,10,14-trimethylhexadeca-5,13-diene-11α-ol(1)、(2 E,10 E)-1,12-dihydroxy-18-acetoxy-3,7,15-trimethylhexadeca-2,10,14-triene(2)、(2 E,6 Z,10 E,12 R)-7-[(acetyloxy)methyl]-3,11,15-trimethylhexadeca-2,6,10,14-tetraene-1,12-diol(3)、(2 E,6 Z,11 S,12 R)-3,7,11,15-tetramethylhexadeca-2,6,14-triene-7-[(acetyloxy)methyl]-1,12,19-triol(4)、plaunotol(5)、S-(+)-dehydrovomifoliol(6)、pubinernoid A(7)、豆甾醇(8)、aurantiamide acetate(9)、反式对羟基肉桂酸(10),其中化合物1为新化合物。采用MTT法对人乳腺癌细胞MDA-MB-231进行细胞毒活性,结果表明,化合物2有较强的细胞毒活性,其IC_(50)值为6.80μmol/L,化合物1、3和4有中等细胞毒活性。
To study the chemical constituents of the medicinal plant Carpesium cernuum and their cytotoxic activity,ten compounds were obtained from the ethyl acetate fraction of 95%ethanol extract of C.cernuum by ap-plying various chromatographic techniques.Their structures were elucidated and identified as(5 Z)-6-[(acetyloxy)methyl]-2-oxo-2,10,14-trimethylhexadeca-5,13-diene-11α-ol(1),(2 E,10 E)-1,12-dihydroxy-18-acetoxy-3,7,15-trimethylhexadeca-2,10,14-triene(2),(2 E,6 Z,10 E,12 R)-7-[(acetyloxy)methyl]-3,11,15-trimethylhexadeca-2,6,10,14-tetraene-1,12-diol(3),(2 E,6 Z,11 S,12 R)-3,7,11,15-tetramethylhexadeca-2,6,14-triene-7-[(acetyloxy)methyl]-1,12,19-triol(4),plaunotol(5),S-(+)-dehydrovomifoliol(6),pubinernoid A(7),stigmasterol(8),aurantiamide acetate(9),E-p-hydroxycinnamic acid(10)respectively,based on the spectral data.Compound 1 is a new compound.The cytotoxic effect of compounds 1-4 on Human breast cancer MDA-MB-231 cell were investigated by MTT.Compound 2 exhibited strong cytotoxic activity with IC_(50) values of 6.80μmol/L,and compounds 1,3 and 4 had moderate cytotoxic activity.
作者
冯树慧
张卫青
孙茂
郭洪位
孙贵萍
朱成光
王天元
晏晨
FENG Shu-hui;ZHANG Wei-qing;SUN Mao;GUO Hong-wei;SUN Gui-ping;ZHU Cheng-guang;WANG Tian-yuan;YAN Chen(Guizhou University of Traditional Chinese Medicine,Guiyang 550002,China;Anshun City People′s Hospital,Anshun 561000,China)
出处
《天然产物研究与开发》
CAS
CSCD
2022年第3期383-389,共7页
Natural Product Research and Development
基金
安顺市科学技术基金(ASKS(2020)07)
安顺市科技创新平台建设计划(安市科平(2019)03号)
贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY-2020-088)
贵州省卫生健康委员会科学技术基金(gzwkj2021-446)。
关键词
烟管头草
化学成分
结构鉴定
细胞毒活性
Carpesium cernuum
chemical constituent
structural identification
cytotoxicity activity
