摘要
目的探究人参皂苷Rg1对H_(2)O_(2)诱导的N2a细胞的保护作用及机制。方法实验分为对照组、模型组、给药组,通过500μmol/L H_(2)O_(2)孵育5 h构建体外细胞损伤模型,给药组给予50μmol/L Rg1。采用CCK-8法检测细胞存活率,运用Hoechst 33258染色法及免疫荧光化学法检测细胞凋亡率,选用试剂盒检测各组细胞LDH、SOD和MDA水平,利用免疫荧光化学法和Western Blot法检测Nrf2和HO-1蛋白的表达情况。结果与对照组相比,模型组细胞的存活率降低(P<0.01),凋亡率、LDH、MDA水平升高(P<0.01),SOD水平和Nrf2、HO-1蛋白表达量下降(P<0.05或P<0.01);而给予50μmol/L Rg1预保护24 h后,存活率明显升高(P<0.05),凋亡率、LDH、MDA水平明显降低(P<0.05或P<0.01),SOD水平和Nrf2、HO-1蛋白表达量明显上升(P<0.05或P<0.01)。结论人参皂苷Rg1可通过激活Nrf2/HO-1信号通路,减轻H_(2)O_(2)诱导的N2a细胞的氧化应激损伤,发挥神经保护作用。
Objective To investigatethe protective effect and mechanism of Ginsenoside Rg1 on H_(2)O_(2)-induced N2a cells.Methods The experiments are divided into control group,model group and drug treatment group.The cell injury mode in vitro is constructed by incubation with 500μmol/L H_(2)O_(2) for 5 h.The drug treatment group is given 50μmol/L Rg1.The cell survival rate is detected by CCK-8,and theapoptosisrate is detected by Hoechst 33258 and immunofluorescence chemistry.The levels of LDH,SOD and MDA are measured by the kits,and the expression of Nrf2 and HO-1 proteins are detected by immunofluorescence assay and Western Blot.Results Compared with the control group,the survival rate of cells in the model group decreased(P<0.01),apoptosis rate,LDH,MDA levels increased(P<0.01),the level of SOD decreased and Nrf2,HO-1 protein expression decreased(P<0.01);while after pre-treating with 50μmol/L Rg1 for 24 h,the survival rate of N2a cells increased(P<0.01),apoptosisrate,LDH,MDA levels decreased(P<0.05,P<0.01),the level of SOD and Nrf2,HO-1 protein expression increased(P<0.05,P<0.01).Conclusion Ginsenoside Rg1 can alleviate H_(2)O_(2)-induced oxidative stress damage in N2a cells and play a neuroprotective role by activating the Nrf2/HO-1signaling pathway.
作者
赵宇萌
陈吉聪
肖洪贺
田雨
李贺
田晋明
杨静娴
ZHAO Yu-meng;CHEN Ji-cong;XIAO Hong-he;TIAN Yu;LI He;TIAN Jin-ming;YANG Jing-xian(Department of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China)
出处
《现代中药研究与实践》
CAS
2022年第1期26-31,共6页
Research and Practice on Chinese Medicines
基金
辽宁省教育厅自然科学基金委员会重点项目(L201945)。
