摘要
该研究采用盆栽法,于百日草幼苗期叶面喷施不同浓度(100、150、200、250和300 mg·kg^(-1))三唑酮(triadimefon,TD)预处理后进行自然干旱和复水处理,分析百日草植株农艺性状及生理特性的变化,并筛选出对百日草抗旱效果最佳的三唑酮浓度,以明确三唑酮对干旱胁迫下百日草幼苗生长的影响机理,为百日草的抗旱栽培应用及抗性品种选育提供理论依据。结果显示:(1)不同浓度的TD预处理能明显提高百日草幼苗的抗旱能力,表现为增加幼苗植株的根长、茎粗、根冠比,降低幼苗的株高,减少叶面积,达到缓解干旱带来的伤害;同时能促进叶绿素(Chl)、可溶性蛋白(SP)和脯氨酸(Pro)含量的增加,提高过氧化物酶(POD)及超氧化物歧化酶(SOD)活性,减缓相对电导率(REC)和丙二醛(MDA)含量的升高。(2)复水后,各处理株高、根长及茎粗均增加,同时,增加Chl、SP、Pro的含量,POD、SOD活性也有所提高,REC和MDA含量逐渐降低。(3)隶属函数分析显示,250 mg·kg^(-1)的三唑酮对百日草的抗旱性促进效果最好。
In this study,the potted method was used.We sprayed triadimefon(TD)with different concentrations(100,150,200,250,300 mg·kg^(-1))on the leaves of Zinnia elegans at the seedling stage,and then treated with natural drought and rehydration.The changes of agronomic and physiological characteristics of Z.elegans were analyzed,and the TD concentration with the best drought resistance effect was also selected to clarify the effect mechanism of TD on the growth of Z.elegans seedlings under drought stress,so as to provide a theoretical basis for the application of drought resistance cultivation and the breeding of resistant varieties.The results showed that:(1)different concentrations of TD pretreatment can significantly improve the drought resistance of Z.elegans seedlings,such as increasing the root length,stem diameter and root shoot ratio of seedlings,reducing the plant height and leaf area,so as to alleviate the damage caused by drought;it can also promote the increase of chlorophyll(Chl),soluble protein(SP)and proline(Pro)contents,improve the activities of peroxidase(POD)and superoxide dismutase(SOD)as well as slow down the increase of relative conductivity(REC)and malondialdehyde(MDA)contents.(2)After rehydration,the plant height,root length and stem diameter of each treatment increased,the contents of Chl,SP and Pro,and activities of POD and SOD increased,while REC and MDA contents decreased gradually.(3)Subordinate function analysis showed that TD with a concentration of 250 mg·kg^(-1) had the best drought resistance effect on Z.elegans seedling.
作者
齐颖
罗平
胡小京
QI Ying;LUO Ping;HU Xiaojing(College of Agriculture, Guizhou University, Guiyang 550025, China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2022年第2期293-300,共8页
Acta Botanica Boreali-Occidentalia Sinica
基金
贵阳市科技计划项目(筑科合同[2019]5-3号,筑科合同[2021]3-5号)。
关键词
百日草
三唑酮
干旱胁迫
生长
生理特性
Zinnia elegans
triadimefon
drought stress
growth
physiological characteristics
