摘要
背景:基质金属蛋白酶7(MMP-7)是一种通过调节多种细胞外基质和非基质底物来参与伤口愈合、组织重塑等过程的蛋白水解酶,是炎症性肠病(IBD)炎症期间组织损伤的重要介质。目的:探讨MMP-7在IBD中的临床意义。方法:纳入2019年1月—2021年1月郑州大学第二附属医院收治的IBD患者结肠活检标本共38例,其中溃疡性结肠炎(UC)20例、克罗恩病(CD)18例,选取5例结肠癌患者癌旁组织作为对照。采用免疫组化染色检测结肠组织中MMP-7表达,并分析其与C反应蛋白(CRP)、白细胞(WBC)的相关性。结果:与对照组相比,活动期UC组和重度CD组MMP-7表达均明显上调(P<0.05)。随着严重程度的增加,MMP-7表达呈逐渐上调的趋势,重度UC和CD组中MMP-7表达与相应缓解期相比明显上调(P<0.05)。不同性别、年龄以及是否使用抗TNF-α制剂的IBD患者之间MMP-7表达差异无统计学意义(P>0.05)。活动期IBD患者结肠组织MMP-7表达与CRP、WBC呈正相关(P<0.001)。结论:MMP-7表达与IBD的活动性和严重程度有一定相关性,与性别、年龄、有无抗TNF-α制剂治疗无关。MMP-7在一定程度上可反映肠道炎症程度,可能是IBD炎症活动的重要指标。
Background:Matrix metalloproteinase-7(MMP-7)is a proteolytic enzyme involved in wound healing,tissue remodeling by regulating a variety of extracellular matrix and non-matrix substrates,and is an important mediator of tissue damage during inflammation in inflammatory bowel disease(IBD).Aims:To investigate the clinical significance of MMP-7 in IBD.Methods:A total of 38 cases of colon biopsy samples from patients with IBD from January 2019 to January 2021 at the Second Affiliated Hospital of Zhengzhou University were collected,including 20 ulcerative colitis(UC)and 18 Crohn’s disease(CD).The paracancerous tissues of 5 patients with colon cancer were served as the control group.The expression of MMP-7 in colon tissue was detected by immunohistochemical staining,and its correlation with C-reactive protein(CRP)and white blood cell(WBC)was analyzed.Results:Compared with the control group,the expression of MMP-7 was significantly up-regulated in both active UC group and severe CD group(P<0.05).The expression of MMP-7 was gradually up-regulated with the increase of severity.The expression of MMP-7 in severe UC and CD group was significantly increased than that in remission UC and CD group(P<0.05).No significant difference in the expression of MMP-7 was found between patients with different gender,age,and whether treated with anti-TNF-αagents(P>0.05).The expression of MMP-7 in colon tissue of patients with active IBD was positively correlated with CRP and WBC(P<0.001).Conclusions:The expression of MMP-7 is correlated with the activity and severity of IBD,but not correlated with gender,age,and whether treated with anti-TNF-αagents.To some extent,MMP-7 can indicate the severity of intestinal inflammation,and may be an important indicator of inflammatory activity in IBD.
作者
庄培丽
周璐
朱瑞齐
王丹丹
张哲
冯百岁
ZHUANG Peili;ZHOU Lu;ZHU Ruiqi;WANG Dandan;ZHANG Zhe;FENG Baisui(Department of Gastroenterology, the Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450014)
出处
《胃肠病学》
北大核心
2021年第6期337-341,共5页
Chinese Journal of Gastroenterology
基金
河南省医学科技攻关计划省部共建项目(SBGJ2018042)
河南省医学科技攻关计划联合共建项目(2018020164)
郑州大学前沿交叉研究专项项目(XKZDQY201906)。
