摘要
目的探讨临床分离的耐甲氧西林金黄色葡萄球菌(MRSA)杀白细胞素(pvl)基因亚型的流行及MLST分子分型特征。方法收集非重复MRSA 287株,按照美国疾病预防控制中心的社区获得性耐甲氧西林金黄色葡萄球菌(CA-MRSA)定义分为医院获得性耐甲氧西林金黄色葡萄球菌(HA-MRSA)和CA-MRSA两组。采用PCR方法扩增pvl基因,产物双向测序并分析核苷酸变异情况;pvl基因阳性(pvl^(+))菌株进行多位点序列(MLST)分型并采用eBURST软件进行克隆复合群(CC)分析。结果287株MRSA中,51株为pvl^(+)菌株(17.8%,51/287),其中29株为CA-MRSA(56.9%,29/51),22株为HA-MRSA(43.1%,22/51)。pvl^(+)菌株经双向测序共发现4个位点(527、663、1396和1729)存在核苷酸变异,根据527位点的差异分为H(H1和H2)和R(R1和R2)亚型。其中46株(90.2%,46/51)为H型(6株H1、40株H2),5株(9.8%,5/51)为R型(1株R1、4株R2)。H型主要属于CC59(ST59/ST388)(69.6%,32/46),其次为CC22(ST22)(10.9%,5/46),CC1(ST1/ST188)(6.5%,3/46)、CC88(ST88)(4.3%,2/46)、CC5(ST149)(4.3%,2/46)、CC9(ST9)(2.2%,1/46)和CC30(ST30)(2.2%,1/46);仅在CC59(ST59)、CC88(ST88)和CC5(ST25)型菌株中发现R型。CA株和HA株携带的pvl均以H亚型为主,分别占86.2%(25/29)和95.5%(21/22),两组差异无统计学意义。结论本地区临床分离MRSA携带的pvl以H亚型(H2)为主,主要克隆型别为CC59;CA-MRSA和HA-MRSA携带的pvl亚型没有明显差异。
This study aimed to investigate the prevalence and MLST molecular typing of leukocytocidal gene subtypes in methicillin resistant Staphylococcus aureus(MRSA).A total of 287 MRSA strains were collected and divided into HA-MRSA and CA-MRSA.The pvl gene was amplified by PCR,and products were sequenced and analyzed for nucleotide variation.The pvl gene positive(pvl^(+))strains were genotyped by multi-site sequence typing(MLST),and the clone complex group(CC)was analyzed in eBURST software.A total of 51 strains were pvl^(+)strains(17.8%,51/287),of which 29 strains were CA-MRSA(56.9%,29/51)and 22 strains were HA-MRSA(43.1%,22/51).Four loci(527,663,1396 and 1729)were found to have nucleotide variations by two-way sequencing.According to the differences at 527 loci,which were divided into H(H1 and H2)and R(R1 and R2)subtypes,46 strains(90.2%,46/51)were H type(6 strains H1,40 strains H2),and five strains(9.8%,5/51)were R type(one strain R1,four strains R2).The H type strains mainly belonged to CC59(ST59/ST388;69.6%,32/46),followed by CC22(ST22;10.9%,5/46),CC1(ST1/ST188;6.5%,3/46),CC88(ST88;4.3%,2/46),CC5(ST149;4.3%,2/46),CC9(ST9;2.2%,1/46)and CC30(ST30;2.2%,1/46).Type R was found only in strains CC59(ST59),CC88(ST88)and CC5(ST25).The pvl carried by CA-MRSA and HA-MRSA was mainly subtype H,accounting for 86.2%(25/29)and 95.5%(21/22),respectively.No significant difference was observed between groups.The pvl carried by MRSA in this region was mainly H subtype(H2),The primary clone type was CC59.No significant difference in PVL subtypes carried by CA-MRSA and HA-MRSA was found.
作者
毛剑锋
张传领
楚旭
MAO Jian-feng;ZHANG Chuan-ling;CHU Xu(Affiliated Xiaoshan Hospital,Hangzhou Normal University,Hangzhou 311201,China)
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2022年第3期242-246,共5页
Chinese Journal of Zoonoses
基金
浙江省医药卫生科技计划项目(No.2019RC256)。
