摘要
为优化黄淮麦区小麦赤霉病抗性鉴定方法,于2020年在河南农业大学许昌校区试验田对4个小麦品种进行单花滴注赤霉病抗性鉴定,分析不同套袋保湿天数对病情严重度的影响,并利用与主效抗病基因Fhb1连锁的功能标记TaHRC-STS对其进行分子检测。结果显示:抗病品种‘苏麦3号’‘宁麦9号’携带该基因,而感病品种不携带;套袋1~7 d处理均引起赤霉病菌侵染,病情严重度随着套袋时间的延长而显著增加,在套袋4~7 d处理下,同一抗、感品种的严重度间无显著差异;且感病品种4~7 d的严重度显著高于1~3 d处理(P<0.05),抗性评价也与1~3 d不同,这表明Fhb1基因能增强小麦赤霉病抗性。进行赤霉病抗性鉴定时,黄淮麦区以套袋保湿4 d的处理效果较好。
In order to optimize the identification method of Fusarium head blight(FHB)resistance in Yellow and Huai River Valley wheat region of China,the FHB resistance of four wheat cultivars planted in experimental field of Xuchang campus of Henan Agricultural University were identified by single floret injection in 2020.The effects of different moisturizing days on the disease severity were analyzed,and then molecular analysis of the four wheat cultivars was performed using TaHRC-STS,a functional marker linked to Fhb1 gene of FHB resistance.The results indicated that resistant cultivars‘Sumai 3’and‘Ningmai 9’carried Fhb1 gene,while susceptible cultivars did not carry it,and the disease severity increased significantly with the extension of bagging time,and there was no significant difference between the same resistant and susceptible cultivars under bagging for four days to seven days.The severity of susceptible cultivars in 4-7 days was significantly higher than that in 1-3 days,and the evaluation of resistance was also different from that in 1-3 days,which indicated that Fhb1 gene could enhance the FHB resistance.In the identification of FHB resistance,bagging and moisturizing for four days was better in Yellow and Huai River Valley wheat region of China.
作者
张根源
胡润雨
陈向果
张漪
许豪
于士男
高艳
李巧云
唐建卫
殷贵鸿
ZHANG Genyuan;HU Runyu;CHEN Xiangguo;ZHANG Yi;XU Hao;YU Shinan;GAO Yan;LI Qiaoyun;TANG Jianwei;YIN Guihong(College of Agronomy,National Key Laboratory of Wheat and Maize Crop Science,Collaborative Innovation Center of Henan Grain Crops,National Engineering Research Center for Wheat,Henan Agricultural University,Zhengzhou450046,China)
出处
《植物保护》
CAS
CSCD
北大核心
2022年第2期208-213,共6页
Plant Protection
基金
中原科技创新领军人才项目(204200510029)
2020年度河南省重大科技专项(201300110800)
河南农业大学拔尖人才项目(30500678)。
关键词
小麦
赤霉病
保湿时间
禾谷镰刀菌
抗性鉴定
wheat
Fusarium head blight
moisturizing time
Fusarium graminearum
resistance identification
