摘要
[目的]明确培养条件(碳源、氮源和pH值)对木腐真菌生长的影响,为延长沙柳(Salix psammophila)沙障使用周期提供理论依据。[方法]以沙柳沙障沙埋段5种木腐真菌:桔绿木霉(Trichoderma citrinoviride)、Xs木霉(Xylogone sphaerospora)、哈茨木霉(Trichoderma harzianum)、拟康氏木霉(Trichoderma koningiopsis)、黑曲霉(Aspergillus niger)为研究对象,在固体和液体下进行培养,分别探究不同培养条件下菌丝生长速度和生物量,确定菌株生长的最适条件。[结果](1)在固体培养条件下,桔绿木霉、Xs木霉、哈茨木霉和黑曲霉以葡萄糖为碳源时生长最快,其生长速度分别为12.97,8.56,13.25,5.22 mm/d;桔绿木霉最佳氮源为硫酸铵13.06 mm/d,Xs木霉、哈茨木霉和拟康氏木霉最佳氮源为蛋白胨,其生长速度分别为9.44,13.17,8.78 mm/d;5种木腐真菌最适pH值在5~7之间。(2)在液体培养条件下,葡萄糖是桔绿木霉、Xs木霉、哈茨木霉、拟康氏木霉和黑曲霉的最佳碳源,每100 ml的培养液中生物量分别达到446.67,454.67,728.67,687.67,713.00 mg;桔绿木霉、Xs木霉、哈茨木霉和拟康氏木霉最适氮源为蛋白胨,黑曲霉以氯化铵为氮源时生物量最大为610.00 mg;5种木腐真菌在pH值5~7的范围内生物量较大。[结论]固体与液体条件下桔绿木霉、Xs木霉和哈茨木霉最适碳源为葡萄糖,最适氮源为蛋白胨,pH值为5~6;拟康氏木霉液体培养条件下最适碳源为葡萄糖,最适氮源为蛋白胨,pH值为5~6;黑曲霉在液体培养条件下最适碳源为葡萄糖、最适氮源为氯化铵、pH值为5~6。
[Objective]The influence of culture conditions(carbon source,nitrogen source,and pH value)on the growth of wood-rot fungi was analyzed in order to provide a theoretical basis for prolonging the service period of Salix psammophila sand barriers.[Methods]Trichoderma citrinovicide,Xylogone sphaerospora,Trichoderma harzianum,Trichoderma koningiopsis,and Aspergillus niger in sand-buried sections of S.psammophila were the five fungi species used as research objects.The growth rate and biomass of mycelium under different culture conditions(solid and liquid)were investigated to determine the optimal growth conditions for each fungi species.[Results]①Under solid culture conditions,T.citrinoviride,X.sphaerospora,T.harzianum,and A.niger grew fastest when glucose was used as the carbon source,and their growth rates were 12.97,8.56,13.25,and 5.22 mm/d,respectively;The best nitrogen source for T.citrinoviride was ammonium sulfate,and the growth rate was 13.06 mm/d.The best nitrogen source for X.sphaerospora,T.harzianum,and T.koningiopsis was peptone,and their growth rates were 9.44,13.17,and 8.78 mm/d,respectively;The optimum pH value for the five species of wood-rot fungi was between 5 and 7.②Under liquid culture conditions,glucose was the best carbon source for the five species of wood-rot fungi,and the biomass values per 100 ml of culture medium reached 446.67,454.67,728.67,687.67,and 713.00 mg,respectively.The most suitable nitrogen source for T.citrinoviride,X.sphaerospora,T.harzianum,and T.koningiopsis was peptone.The biomass of A.niger was 610.00 mg when ammonium chloride was used as the nitrogen source.The biomass of the five species of wood-rot fungi was large in the pH value range from 5 to 7.[Conclusion]Under solid and liquid conditions,the most suitable carbon source for T.citrinoviride,X.sphaerospora,and T.harzianum is glucose,the most suitable nitrogen source is peptone,and the pH value is between 5 and 6.Under the liquid culture condition,the most suitable carbon source for T.koningiopsis is glucose,the most suitable nitrogen source is peptone,and the pH value is between 5 and 6.Under the liquid culture condition,the most suitable carbon source for A.niger is glucose,the most suitable nitrogen source is ammonium chloride,and the pH value is between 5 and 6.
作者
段晓婷
高永
梁钰镁
王瑞东
赵晨
郭鑫
Duan Xiaoting;Gao Yong;Liang Yumei;Wang Ruidong;Zhao Chen;Guo Xin(College of Desert Control Science and Engineering,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010020,China)
出处
《水土保持通报》
CSCD
北大核心
2022年第1期93-98,共6页
Bulletin of Soil and Water Conservation
基金
国家自然科学基金项目“大气—沙土界面动态过程对沙柳沙障耐蚀性的强化机制”(41861044)。
关键词
木腐真菌
碳源
氮源
酸碱度
沙柳
沙障
wood-rotting fungi
carbon source
nitrogen source
pH value
Salix psammophila
sand barrier
