摘要
为了快速鉴定二倍体马铃薯种质资源的耐盐性,本研究以17份二倍体马铃薯无性系试管苗为供试材料,测定5个形态指标和8个生理生化指标,并通过主成分分析、隶属函数分析、聚类分析及逐步回归分析对17个无性系进行耐盐性综合评价。结果表明:主成分分析将5个形态指标简化为1个独立的综合指标,将8个生理生化指标简化为4个独立的综合指标;17个无性系被聚类分析为3种耐盐类型,其中耐盐无性系包括A038、A002和A024;利用形态指标建立了耐盐性评价的回归方程;可用于离体快速鉴定二倍体马铃薯耐盐性的指标为芽长、芽干重、根鲜重、根干重、相对含水量、丙二醛含量和脯氨酸含量。本研究可为马铃薯离体耐盐性评价提供参考依据,并为马铃薯耐盐新品种选育提供种质资源。
In order to quickly evaluate the salt tolerance of diploid potato germplasm resources,five morphological indices and eight physiological and biochemical indices of plantlets in vitro of 17 diploid potato clones were measured using principal component analysis,subordinate function analysis,clustering analysis,and stepwise regression.The five morphological indices were simplified into one independent comprehensive index,while the eight physiological and biochemical indices were simplified into four independent comprehensive indices using principal component analysis.Those seventeen clones were classified into three clusters with different salt tolerance using cluster analysis,with A038,A002,and A024 being classified into the salt tolerance cluster.A regression equation of salt tolerance evaluation was established by morphological index.Shoot length,shoot dry weight,root fresh weight,root dry weight,relative water content,MDA content,and proline content could be used to quantify salt tolerance of potato in vitro.This study provides reference for evaluating salt tolerance of potato in vitro,and provides germplasm resources for breeding salt tolerant potato varieties.
作者
赵明辉
白雅梅
孟令波
孙婷婷
王德权
景重阳
吕文河
ZHAO Ming-hui;BAI Ya-mei;MENG Ling-bo;SUN Ting-ting;WANG De-quan;JING Chong-yang;LYU Wen-he(College of Geography and Tourism,Harbin University,Harbin 150086,China;College of Agriculture,Northeast Agricultural University,Harbin 150030,China;College of Resources and Environment,Northeast Agricultural University,Harbin 150030,China;College of Food Engineering,Harbin University,Harbin 150086,China)
出处
《生态学杂志》
CAS
CSCD
北大核心
2022年第3期610-617,共8页
Chinese Journal of Ecology
基金
黑龙江省马铃薯产业技术协同创新推广体系(2021)
黑龙江省自然科学基金联合引导项目(LH2019C041)
哈尔滨学院大学生创新创业训练计划项目(201910234048)资助。
关键词
马铃薯
二倍体
盐胁迫
主成分分析
隶属函数
逐步回归
potato
diploid
salt stress
principal component analysis
subordinate function
stepwise regression
