摘要
目的:用斑蝥素诱导SD大鼠肝脏损伤并建立肝脏毒性模型,研究人参皂苷Rb1对SD大鼠肝脏毒性的保护作用。方法:取SD大鼠40只随机分为正常组、斑蝥素组、人参皂苷Rb1低、高剂量组,每组10只。用斑蝥素连续15d腹腔注射给药,建立SD大鼠肝毒性模型;用人参皂苷Rb1低、高剂量提前4d腹腔注射干预给药,第5天至第19天每天提前1h给予人参皂苷Rb1低、高计量组腹腔注射干预后再用斑蝥素腹腔注射给药。通过血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、肝脏HE染色考察人参皂苷Rb1对肝脏的保护作用;Westrn blot法考察肝脏中MAPK通路有关蛋白的变化。结果:与正常组比较,斑蝥素组血清ALT、AST显著上升(P<0.01),HE结果显示肝小叶结构模糊、肝索絮乱,p-ERK、p-JNK、p-p38MAPK蛋白显著下调(P<0.01);与斑蝥素比,人参皂苷Rb1低、高剂量组血清中ALT、AST的含量明显减少至近正常组水平(P<0.05,P<0.01),明显改善肝组织受损情况;低剂量组显著上调p-ERK、p-JNK、p-p38 MAPK蛋白的表达(P<0.05,P<0.01)。结论:人参皂苷Rb1对斑蝥素所致SD大鼠肝脏毒性有一定的保护作用,可能和上调MAPK家族成员p-ERK、pJNK、p-p38MAPK有关。
Objective:To investigate the protective effect of ginsenoside Rb1 on cantharidin-induced liver toxicity in SD rats.Objective:The 40 SD rats were randomly divided into normal group,cantharidin group,ginsenoside Rb1 low dose group and high dose group with 10 rats in each group.The model of liver toxicity were established by continous injection cantharidin intraperitoneally for 15 days,ginsenoside Rb1 group were administered through intraperitoneal injection for 4 days,from the 5 th day to the 19 th day,the low dose group and the high dose group were given the corresponding doses of ginsenoside Rb1 respectively at 1 hbefore being given cantharidin,the drugs were given once every day.The protective effects of ginsenoside Rb1 on liver tissue injuries caused by cantharidin was observed by using hematoxylineosin staining and tested the levels of serum AST,ALT;MAPK family protein levels of p-ERK,p-JNK,p-p38 in liver tissues were detected by Western blot.Results:Compared with the normal group,the contents of AST,ALT were obviously increased(P<0.01);the structure of rat’s liver lobule was border blurry and the hepatic cord was disordered;the expressions of p-ERK,p-JNK,p-p38 MAPK in liver tissues were significantly down-regulated(P<0.01).Compared with the canthadridin,ginsenoside Rb1 could dramatically decrease the levels of of serum AST,ALT(P<0.05,P<0.01);ameliorated the levels of liver injury;low dose treatment group significantly up-regulate the protein expression of could ameliorate the levels of p-ERK,p-JNK,p-p38 MAPK(P<0.05,P<0.01).Conclusion:Ginsenoside Rb1 exer protective effect against cantharidin-induced liver toxicity by up-regulating pERK,pJNK,p-p38 MAPK signal pathway.
作者
胡超
刘艳丽
许琼明
李笑然
杨世林
Hu Chao;Liu Yanli;Xu Qiongming;Li Xiaoran;Yang Shilin(Tongren Polytechnic College,Tongren 554300,China;Soochow University,Suzhou 215123,China)
出处
《亚太传统医药》
2022年第3期8-11,共4页
Asia-Pacific Traditional Medicine
基金
国家自然科学基金(81573548)
铜仁市科技局科技计划面上项目(铜市科研(2019)12-8号)
铜仁职业技术学院科研项目(tzky-2018年-zk14号)。
