摘要
目的基于网络药理学方法分析加味柴苓汤治疗高脂血症性胰腺炎的作用机制,为临床的合理使用、动物实验、新药研发提供依据。方法通过中医药系统药理学数据库及分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)数据库获取半夏、柴胡、党参、黄芩、生姜、大枣、甘草、猪苓、茯苓、泽泻、苍术、桂枝、厚朴、薏苡仁、丹参、陈皮的主要化学成分及其对应靶点;通过GenCards数据库获取高脂血症性胰腺炎的疾病靶点;利用Venny网站获取共同靶点。以Cytoscape3.7.2系统构建"疾病-中药-活性成分-靶点"网络图。通过STRING平台构建"PPI"网络图,进行蛋白质相互作用分析。采用Metascape平台进行基因本体(GO)和基因相互之间(KEGG)富集分析,并构建"靶点-通路"网络图以筛选重要通路,最终获取主要作用的关键靶点。结果加味柴苓汤治疗高脂血症性胰腺炎的主要活性成分为槲皮苷、柚皮素、木犀草素、山柰酚、汉黄芩素等,关键靶点包括AKTI、TL6、PPARG、TNF、CASP3、NOS2等,主要的生物过程为脂质定位、类固醇代谢过程、脂质代谢过程的调节、对脂多糖的反应、活性氧代谢过程等;主要作用于脂质结合、细胞因子活性、丝氨酸型肽酶活性、辅因子结合、类固醇结合等分子功能;主要富集的通路有:糖尿病并发症中的AGE-RAGE信号通路、非酒精性脂肪性肝病、癌症的途径、缺氧诱导因子-1信号通路、PPAR信号通路、MAPK信号通路等。结论研究初步揭示加味柴苓汤治疗高脂血症性胰腺炎是多药物、多成分、多靶点、多通路共同参与作用的结果,为日后加味柴苓汤的临床使用提供了一定的科学依据。
Objective To analyze the mechanism of Modified Chailing Decoction(柴苓汤)in the treatment of hyperlipidemic pancreatitis based on network pharmacology,so as to provide basis for clinical rational use,animal experiment and new drug research and development.Methods Traditional Chinese Medicine Systems Pharmacy Database and Analysis Platform(TCMSP)database was used to obtain the main chemical constituents and corresponding targets of Banxia(Pinelliae Rhizoma),Chaihu(Bupleuri Radix),Dangshen(Codonopsis Radix),Huangqin(Scutellariae Radix),Shengjiang(Zingiberis Rhizoma Recens),Dazao(Jujubae Fructus),Gancao(Glycyrrhizae Radix Et Rhizoma),Zhuling(Polyporus),Fuling(Poria),Zexie(Alismatis Rhizoma),Cangzhu(Atractylodis Rhizoma),Guizhi(Cinnamomi Ramulus),Houpo(Magnoliae Officinalis Cortex),Yiyiren(Coicis Semen),Danshen(Salviae Miltiorrhizae),Chenpi(Citri Reticulatae Pericarpium).The disease targets of hyperlipidemic pancreatitis were obtained from gencards database,and the common targets were obtained from Venny website.The“disease traditional Chinese medicine active ingredients target”network diagram was constructed with Cytoscape 3.7.2 to screen the main active ingredients.String platform was used to analyze protein-protein interaction,and“PPI”network was constructed.Go and KEGG enrichment analysis was carried out by using metascape platform,and the“target pathway”network diagram was constructed to screen important pathways,and finally the key targets were screened.Results The main active components of Modified Chailing Decoction in the treatment of hyperlipidemic pancreatitis are quercetin,naringenin,luteolin,kaempferol,wogonin and so on.The key targets are AKTI,TL6,PPARG,TNF,CASP3,NOS2 and so on.The main biological processes include lipid localization,steroid metabolism,regulation of lipid metabolism,response to lipopolysaccharide,active oxygen metabolism and so on.It acts on lipid binding,cytokine activity,serine peptidase activity,cofactor binding,steroid binding and other molecular functions;the main enriched pathways are AGE-RAGE signaling pathway in diabetic complications,nonalcoholic fatty liver disease,cancer pathway,hypoxia inducible factor-1 signaling pathway,PPAR signaling pathway,MAPK signaling pathway,etc.Conclusion This study preliminarily revealed the multi-component,multi-target and multi-channel mechanism of Modified Chailing Decoction in the treatment of hyperlipidemic pancreatitis,and provided scientific basis for the clinical use of Modified Chailing decoction.
作者
李涛
张商傲
高允海
LI Tao;ZHANG Shang'ao;GAO Yunhai(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China;Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,Liaoning,China)
出处
《辽宁中医药大学学报》
CAS
2022年第1期67-74,F0003,共9页
Journal of Liaoning University of Traditional Chinese Medicine
基金
科技部国家重点研发计划(2018YFE0195200)。
关键词
柴苓汤
网络药理学
高脂血症性胰腺炎
靶点预测
富集分析
Chailing Decoction(柴苓汤)
network pharmacology
hyperlipidemic pancreatitis
target prediction
enrichment analysis
