摘要
以乙醇为浸提剂,采用球磨法提取花生壳中的黄酮类物质,考察了球磨时间、料液比和乙醇浓度对总黄酮提取量的影响,在单因素实验基础上,采用Box-Behnken设计优化总黄酮提取工艺,并研究了花生壳黄酮提取物的抗氧化能力。结果表明,花生壳黄酮的最佳提取条件为:乙醇浓度60%,料液比1:25(g/mL),球磨时间60 min,花生壳黄酮的提取量为(32.1±0.13)mg/g。当花生壳黄酮浓度为0.012 mg/mL时,对超氧自由基阴离子的清除率为53.53%。表明球磨法是一种有效的花生壳黄酮提取方法,为进一步研究开发花生壳资源提供了一定的理论数据支持。
Flavonoids in peanut hull were extracted by the ball milling method.The effects of solid-liquid ratio,ball milling time,and ethanol volume fraction on the extraction yield of flavonoids were investigated by single factor experiments.The Box-Behnken response surface methodology was used to optimize the extraction process,and the scavenging rate of superoxide free radical was also studied.The results showed that the optimal extraction conditions were as follows:Solidliquid ratio of 1:25 g/mL,ball milling time of 60 min,and ethanol concentration of 60%,and the extraction yield of flavonoids could reach(32.1±0.13)mg/g.The scavenging capacity of superoxide free radicals was 53.53% when the concentration of flavonoids was 0.012 mmol/L.This work proved that ball milling was an effective extraction method and would provide the theoretical data support for further research.
作者
吕东灿
侯婧霞
姜广策
李演鑫
李程鹏
王志敏
LV Dongcan;HOU Jingxia;JIANG Guangce;LI Yanxin;LI Chengpeng;WANG Zhimin(College of Sciences,Henan Agricultural University,Zhengzhou 450002,China;Shangqiu Hospital of TCM,Shangqiu 476000,China)
出处
《食品工业科技》
CAS
北大核心
2022年第8期212-218,共7页
Science and Technology of Food Industry
基金
河南省科技攻关项目(202102310267,192102310220)
河南农业大学科研启动项目(30601332,KJCX2019A20)
河南省高校科技创新人才支持计划(18HASTIT038)。
关键词
花生壳
球磨
黄酮提取
工艺优化
抗氧化活性
peanut hull
ball milling
flavonoids extraction
process optimization
antioxidant activity
