摘要
设置不同培养料配方(麦粒含量分别为0、25%、50%、75%和100%,其余为木屑,命名为M0、M1、M2、M3和M4)和不同种袋放置数量(8、12、14和16)处理组合20个,栽培3个六妹羊肚菌(Morchella sextelata)菌株YAASJNLM1-31、YAASJNLM6-6和YAASJNLM6-20,测定产量;测定菌株YAASJNLM1-31接种前和采收后种袋基质的总干重、有机碳和总氮含量,探索种袋栽培条件下种袋基质消耗、有机碳消耗和总氮消耗与羊肚菌产量之间的关系。结果表明:对于菌株YAASJNLM1-31,种袋配方为M1时,种袋放置数量为16时的产量较高;种袋配方为M4时,种袋放置数量为12、14和16时的产量较高;种袋放置数量为14和16时,配方M1~M4的产量较高。对于菌株YAASJNLM6-6,种袋放置数量为8时,配方M2~M4的产量较高;种袋放置数量为12时,配方M1~M4的产量较高;种袋放置数量为14时,配方M2、M3的产量较高。对于菌株YAASJNLM6-20,种袋放置数量为8时,配方M1~M4产量较高。对于菌株YAASJNLM1-31,不同处理种袋基质消耗比例(40.81%~64.75%)差别较大,纯木屑配方、纯麦粒配方种袋基质消耗比例分别为40.81%~41.73%、49.65%~50.44%;种袋基质有机碳消耗和总氮消耗均与产量显著正相关。研究结果为优化羊肚菌种袋栽培方法提供参考。
A novel morel cultivation mode using spawn bottles was developed. In the new mode, mycelial blocks were inoculated into transparent glass jars(560 mL each) filled with cultivation substrate(spawn bottle), cultivated at 18 ℃ in darkness for 3 d to allow mycelial growth, and then the spawn bottles were placed on planting land with the mouth down on the ground and cultivated until harvest. Different cultivation substrates in combination with different spawn bottle numbers per cultivation plot of 1.5 m×1.5 m were studied on three strains of Morchella sextelata, YAASJNLM1-31, YAASJNLM6-6 and YAASJNLM6-20, respectively. Different cultivation substrates comprised different proportions of wheat grain and sawdust, including 100% sawdust(M0), 25% wheat grain+75% sawdust(M1), 50% wheat grain+50% sawdust(M2), 75% wheat grain+75% sawdust(M3) and 100% wheat grain(M4), respectively. Different spawn bottle placements were 8, 12, 14 and 16 bottles per cultivation plot of 1.5 m×1.5 m, respectively. Each strain was cultivated in all 20 combinations of the above conditions, and then measured for yield and nutrient consumption in spawn bottles. The results showed that the yield of YAASJNLM1-31 was high using M1 and 16 bottles per cultivation plot. When cultivated on M4, the yield of YAASJNLM1-31 was high under 12, 14 and 16 bottles per cultivation plot. When 14 or 16 bottles were placed on a cultivation plot, the yield of YAASJNLM1-31 was high on M1-M4. For YAASJNLM6-6, high yields were obtained under the following combinations: M2×8 bottles per cultivation plot, M3×8 bottles per cultivation plot, M4×8 bottles per cultivation plot, M1×12 bottles per cultivation plot, M2×12 bottles per cultivation plot, M3×12 bottles per cultivation plot, M4×12 bottles per cultivation plot, M2×14 bottles per cultivation plot, and M3×14 bottles per cultivation plot. For YAASJNLM6-20, high yields were obtained under M1-M4 with 8 bottles per cultivation plot. For YAASJNLM1-31, the substrate consumption ratio of each treatment group varied greatly(40.81%-64.75%). The consumption ratio of YAASJNLM1-31 on M0 was 40.81%-41.73%, and the consumption ratio of YAASJNLM1-31 on M4 was 49.65%-50.44%. Both organic carbon consumption and total nitrogen consumption were positively correlated with yield. The above results provided a reference to improve morel cultivation mode.
作者
向刚
马渊浩
刘萍
李仔密
柴红梅
赵永昌
XIANG Gang;MA Yuanhao;LIU Ping;LI Zimi;CHAI Hongmei;ZHAO Yongchang(School of Agriculture,Yunnan University,Kunming 650504,Yunnan,China;Biotechnology and Genetic Germplasm Resource Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205,Yunnan,China)
出处
《食用菌学报》
CSCD
北大核心
2022年第2期39-47,共9页
Acta Edulis Fungi
基金
国家现代农业产业技术体系建设专项(CARS-20)
国家自然科学基金(31960621)。
关键词
六妹羊肚菌
种袋
配方
产量
碳氮比
Morchella sextelata
spawn bottle
formula
yield
C∶N ratio
