摘要
目的:探讨肝纤维化病理过程中肝组织蛋白酪氨酸磷酸酶SHP1的动态表达。方法:50只SD大鼠随机分为对照组(n=10)和模型组(n=40),大鼠肝纤维化模型采用腹腔注射四氯化碳法构建,Masson三色及HE染色测定大鼠肝脏组织的病理学改变,Western blot、免疫组织化学染色和Real-time PCR测定大鼠肝组织的SHP1表达。结果:免疫组织化学染色显示,正常大鼠肝组织表达少量SHP1,主要位于细胞浆,且随大鼠肝纤维化的加重,肝组织SHP1表达逐渐增加(P<0.05),主要分布于纤维化区域及汇管区。Western blot及Real-time PC检测显示,造模不同时间(2、4、6、8周)大鼠肝组织中SHP1蛋白表达(相对积分光密度值分别为0.53±0.04、0.73±0.04、0.87±0.03、1.10±0.07)及mRNA表达(分别为对照组的1.14、1.36、1.49及1.61倍)均高于对照组(相对积分光密度值为0.35±0.07,mRNA表达为1,P<0.05),并随肝纤维化的加重逐渐增加(P<0.05)。结论:大鼠肝纤维化病理过程中肝组织的SHP1蛋白及mRNA表达均逐渐升高,且升高程度与肝纤维化程度一致。
Objective:To investigate the dynamic expression of protein tyrosine phosphatase SHP1 in liver tissue during the pathological process of liver fibrosis.Methods:50 male healthy SD rats were randomly divided into model group(n=40)and control group(n=10).Through using intraperitoneal injection of carbon tetrachloride,rats model of hepatic fibrosis was constructed.Hematoxylin and eosin(HE)and Masson’s trichrome staining were used to observe the histological changes of rats liver tissues.SHP1 expression of liver tissues in rats was analyzed through Western blot,immunohistochemical staining,Real-time fluorescent quantitative PCR assay.Results:Immunohistochemical staining showed that there were a small amount of SHP1 expressions in normal rats liver tissues,and mainly located in the cytoplasm.In fibrotic rats liver tissues,SHP1 mainly distributed in fibrosis areas and portal areas,with the aggravation of hepatic fibrosis the expressions of SHP1 gradually increased(P<0.05).Western blot and real time PC showed that the expression of SHP1(relative integrated optical density 0.53±0.04,0.73±0.04,0.87±0.03,1.10±0.07)protein and mRNA(1.14,1.36,1.49 and 1.61 times)in liver tissue of rats at different times(2,4,6,8 weeks)of modeling were higher than those in the control group(relative integrated optical density 0.35±0.07,mRNA expression 1,P<0.05),and gradually increased with the aggravation of liver fibrosis(P<0.05).Conclusion:During the pathological process of hepatic fibrosis in rats,the expression of SHP1 protein and mRNA in liver tissue gradually increased with hepatic fibrosis degree,and the degree of increase is according with severity of liver fibrosis.
作者
靳丽敏
郝礼森
刘玉龄
杨小师
展宗媛
陈盼盼
苗笑佳
何宇
JIN Li-min;HAO Li-sen;LIU Yu-ling;YANG Xiao-shi;ZHAN Zong-yuan;CHEN Pan-pan;MIAO Xiao-jia;HE Yu(Department of Gastroenterology,1.North China University of Science and Technology Affiliated Hospital,Tangshan 063000;The Second Affiliated Hospital of Xingtai Medical College,Xingtai 054000,Hebei,China)
出处
《川北医学院学报》
CAS
2022年第3期275-278,共4页
Journal of North Sichuan Medical College
基金
河北省自然科学基金资助项目(H2018209366)。
