摘要
蓝藻是地球上最古老的生物之一,其形态结构较为简单,为产氧型光合作用的原核生物。山西省晋阳湖为华北地区最大的人工湖,该研究以采自晋阳湖水体及岸边附着的蓝藻为材料,采用经典毛细管法分离纯化出5株丝状蓝藻,利用光学显微镜观察其形态结构特征(如细胞形状、藻丝体宽度、是否有鞘)和显微结构,并采用16S rRNA序列分析其系统发育关系,以明确晋阳湖的蓝藻种类,为预防湖泊蓝藻水华的发生、维护水资源环境稳定与生态平衡提供理论数据。结果显示:(1)所分离纯化的5株丝状蓝藻依形态特征归属于3个科,其中2株(JYH005和JYH012)为细鞘丝藻亚科(Leptolyngbyaceae),2株(JYH008和JYH022)为伪鱼腥藻科(Pseudanabaenaceae),1株(JYH010)为沙丝藻科(Desertifilaceae)。(2)基于16S rRNA序列构建的系统发育树显示,5株丝状蓝藻中JYH005为结丝藻属(Nodosilinea)的一种;JYH008可归为Arthronema,该株蓝藻在培养条件下观察到不同的形态特征,可能为新物种;JYH010为沙丝藻属(Desertifilum)的一种;JYH012可归为细鞘丝藻属(Leptolyngbya);JYH022与伪鱼腥藻科聚为一支,由于与该科其他藻相似度低于90%,且不能聚为一支,因此只能归为伪鱼腥藻科。研究表明,基于16S rRNA序列系统发育分析与形态学鉴定结果相一致。该研究结果丰富了山西省晋阳湖丝状蓝藻的多样性,为该湖的资源利用和环境保护提供了一定的科学依据。
Cyanobacteria is one of the oldest organisms on earth.It is prokaryotes of oxygen-producing photosynthesis with relatively simple morphology.Jinyang Lake is located in Shanxi Province,which is the largest artificial lake in North China.In this study,five filamentous cyanobacteria were isolated and purified by classical capillary method from algae collected from the water body and shore of Jinyang Lake.Their morphological characteristics(such as cell shape,filamentous width,whether there is sheath)and microstructure were observed by optical microscope,and their phylogenetic relationship were analyzed by 16S rRNA sequence for clarifing the cyanobacteria species in Jinyang Lake.It provided theoretical data for preventing the occurrence of cyanobacteria blooms in lake and maintaining the stability of water resources and environment and ecological balance.The results showed that:(1)the five filamentous cyanobacteria belonged to three families according to morphology,among which two strains of filamentous cyanobacteria were Leptolyngbyaceae(JYH005 and JYH012),two strains were Pseudanabaenaceae(JYH008 and JYH022),and one strain was Desertifilaceae(JYH010).(2)According to the phylogenetic tree based on 16S rRNA sequence,JYH005 was Nodosilinea,and JYH008 was classified as Arthronema,but different morphological characteristics were observed under culture conditions,which may be a new species.JYH010 was a type of Desertifilum.JYH012 was classified as Leptolyngbya.JYH022 was grouped with Pseudanabaenaceae,and the similarity with other cyanobacteria of this family was less than 90%.Therefore,JYH022 belonged to a different genus,so it only was grouped into Pseudanabaenaceae.The research suggested that the morphological identification of the isolated cyanobacteria strains was consistent with the results of 16S rRNA sequence phylogenetic analysis.This study enriched the diversity of filamentous cyanobacteria in Jinyang Lake,Shanxi Province,and provided a certain scientific basis for resource utilization and environmental protection of the lake.
作者
肖喆
王捷
石瑛
王清华
李艳晖
谭梅娟
XIAO Zhe;WANG Jie;SHI Ying;WANG Qinghua;LI Yanhui;TAN Meijuan(Department of Biology, Taiyuan Normal University, Jinzhong, Shanxi 030619, China;2Shanxi Vocational University of Engineering Science and Technology, Jinzhong, Shanxi 030619, China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2022年第3期427-434,共8页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(32000167)
山西省应用基础研究计划(201801D221282,201901D211421)
山西省高等学校科技创新项目(2020L0524)
山西省高等学校教学改革项目(J2020273)。
关键词
丝状蓝藻
16S
rRNA
藻类鉴定
晋阳湖
filamentous cyanobacteria
16S rRNA
algae identification
Jinyang Lake
