摘要
以苹果砧木M9T337(Malling 9 NAKBT337)为试材,克隆了1个WRKY转录因子基因,命名为MdWRKY74(XM_029090147.1)。其开放阅读框为939bp,编码312个氨基酸,含有1个典型的WRKY结构域。氨基酸序列比对和进化树分析发现MdWRKY74与梨PyWRKY74同源性最高。亚细胞定位结果显示MdWRKY74定位于细胞核。荧光定量PCR分析表明,MdWRKY74在苹果的不同组织中均有表达。在拟南芥中过表达MdWRKY74可提高植株的抗盐性,并能促进SOS1和NHX1的表达。结果表明MdWRKY74可被盐胁迫诱导,可能参与苹果抗盐调控。
In this study,a WRKY transcription factor gene,named MdWRKY74(XM_029090147.1)was obtained from apple rootstock M9T337(Malling 9 NAKBT337).The open reading frame of MdWRKY74 was 939 bp,which encoded 312 amino acids,and contained a typical WRKY domain.Amino acid sequence alignment and phylogenetic tree analysis showed that MdWRKY74 had the highest homology with Pyrus bretschneideri WRKY74.Subcellular localization assays showed that MdWRKY74was located in the nucleus.Quantitative real-time PCR analysis showed that MdWRKY74 was expressed in different apple tissues.Overexpression of MdWRKY74 in Arabidopsis could improve the salt resistance of plants,and promote the expression of SOS1 and NHX1.The results showed that MdWRKY74 can be induced by salt stress,so it may participate in the regulation of salt resistance in apple.
作者
相立
赵蕾
王玫
吕毅
王艳芳
沈向
陈学森
尹承苗
毛志泉
XIANG Li;ZHAO Lei;WANG Mei;LüYi;WANG Yanfang;SHEN Xiang;CHEN Xuesen;YIN Chengmiao;MAO Zhiquan(State Key Laboratory of Crop Biology,College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an,Shandong 271018,China;Weihai Acedemy of Agricultural Sciences,Weihai,Shandong 264200,China;College of Chemistry and Material Science,Shandong Agricultural University,Tai'an,Shandong 271018,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2022年第3期482-492,共11页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(32072510)
国家现代农业产业技术体系建设专项资金项目(CARS-27)
山东省自然科学基金项目(ZR2020MC131)
山东省农业重大应用技术创新项目(SD2019ZZ008)
泰山学者项目(ts20190923)
山东省高等学校青创科技支持计划项目(2019KJF020)
山东省水果创新团队项目(SDAIT-06-07)。
