摘要
分别测试了丹参挥发油、蒲公英提取物及其复配物的抗氧化、美白、抑菌、细胞毒性和体外皮肤渗透性,考察丹参挥发油与蒲公英提取物复配后是否具有协同增效作用。结果表明,复配物的抗氧化活性比二者单独作用效果更强,略高于阳性对照V_(C);丹参挥发油对酪氨酸酶活性的抑制作用强于阳性对照熊果苷,对酪氨酸酶的抑制类型为竞争性抑制,抑制常数(K_(1))为0.0049 mg/L,丹参挥发油与蒲公英提取物两者之间对酪氨酸酶的抑制无拮抗作用;复配物对金黄色葡萄球菌的抑制作用好于单一成分,与阳性对照硫酸链霉素效果相当;提取物质量浓度为100 mg/L时无明显细胞毒性;丹参挥发油与蒲公英复配后,蒲公英提取物的皮肤累计渗透量比单独作用时增大,丹参挥发油可促进蒲公英提取物的透皮吸收,复配物具有协同抗氧化和抑菌功效,无明显细胞毒性,皮肤渗透性较好。
The antioxidant activity,whitening,antibacterial activity,cytotoxicity and skin permeability in vitro of Salvia miltiorrhiza volatile oil,dandelion extract and their complexes were tested.The synergistic effects of Salvia miltiorrhiza volatile oil and dandelion extract were investigated.The results showed that the antioxidant activity of the complexes was stronger than that of the two alone,slightly higher than that of positive control V_(C).The results of enzyme kinetics showed that the inhibitory effect of Salvia miltiorrhiza volatile oil on tyrosinase activity was stronger than that of arbutin,the positive control.The inhibitory type of Salvia miltiorrhiza volatile oil on tyrosinase was competitive,and the inhibition constant(K_(1))was 0.0049 mg/L.There was no antagonistic effect between Salvia miltiorrhiza volatile oil and dandelion extract on tyrosinase activity.The inhibitory effect of the complexes on Staphylococcus aureus was better than that of the single component,which was similar to positive control,streptomycin sulfate.The extracts with a mass concentration of 100 mg/L had no cytotoxicity.After complexing Salvia miltiorrhiza volatile oil and dandelion extract,the cumulative permeability of dandelion extract was greater than that when acting alone,and Salvia miltiorrhiza volatile oil promoted the transdermal absorption of dandelion extract.The complexes of Salvia miltiorrhiza volatile oil and dandelion extract had synergistic antioxidant and antibacterial effects,no obvious cytotoxicity,better skin permeability.
作者
吴颖
刘晴
唐文
王佳其
王双旦
侯芙蓉
WU Ying;LIU Qing;TANG Wen;WANG Jiaqi;WANG Shuangdan;HOU Furong(School of Chemical and Environmental Engineering,Shanghai Institute of Technology,Shanghai 201418,China;School of Perfume and Aroma Technology,Shanghai Institute of Technology,Shanghai 201418,China)
出处
《精细化工》
EI
CAS
CSCD
北大核心
2022年第3期562-568,共7页
Fine Chemicals
基金
上海市大学生创新训练项目(10110K208035-B11,10110K208031-B11)。
关键词
丹参挥发油
蒲公英
协同增效
抗氧化活性
美白
抑菌活性
细胞毒性
皮肤渗透
Salvia miltiorrhiza volatile oil
dandelion
synergistic effect
antioxidant activity
whitening
antibacterial activity
cytotoxicity
skin permeability
