摘要
利用水稻ROXY1、ROXY2基因序列,在玉米基因组数据库进行同源检索,发现GRMZM2G442791基因(MS22)序列与ROXY1、ROXY2之间具有较高的相似度(73%~79%)。以玉米B73自交系为实验材料克隆MS22启动子区序列;用生物信息学方法对水稻ROXY1、ROXY2及MS22基因核苷酸序列以及蛋白质结构、亲水性和疏水性及进化树等进行分析。结果表明,玉米雄性核不育相关基因MS22编码区长度为480 bp,编码159个氨基酸;ROY1编码区长度为408 bp,编码136个氨基酸;ROXY2编码区长度为420 bp,编码140个氨基酸。通过对蛋白功能结构域进行分析发现,MS22、ROXY1、ROXY2都为谷氧还蛋白家族成员,具有GRX家族基因相似的功能结构域和近似的三级结构。因此推测,MS22可能与ROXY1、ROXY2具有相似的功能,可能参与氧化还原调节、植物胁迫防御,在控制雄性配子发生中起主要调控作用。
Using rice ROXY1 and ROXY2 gene sequences, a homology search was conducted in the maize genome database.the searching results showed that the GRMZM2G442791(MS22) gene sequence had a high degree of similarity with ROXY1and ROXY2(73%-79%). Primers were designed by using Primer5.0, and MS22 was cloned by using the maize B73 inbred line.Bioinformatics methods were used to analyze the nucleotide sequences, protein structure, hydrophilia, hydrophobicity and evolution tree of rice ROXY1, ROXY2 and MS22. The results showed that the coding regions of the maize male sterility related gene MS22, ROY1 and ROXY2 are 480 bp, 408 bp and 420 bp in length, respectively and encode 159 amino acids, 136 amino acids and 140 amino acids, respectively. The analysis of protein functions and domains showed that MS22, ROXY1 and ROXY2are members of the glutaredoxin(GRX) family and have the similar functional domains and similar tertiary structure to GRX family genes. Therefore, it is speculated that MS22 may have similar functions to ROXY1 and ROXY2, could participate in redox regulation, plant stress defense, and play a major role in controlling male gametogenesis.
作者
高永钢
GAO Yonggang(Inner Mongolia University of Science and Technology,Baotou,Inner Mongolia 014010,China;College of Energy and Envi-ronment,Inner Mongolia University of Science and Technology,Baotou,Inner Mongolia 014010,China)
出处
《热带农业科学》
2022年第3期45-50,共6页
Chinese Journal of Tropical Agriculture
基金
内蒙古自治区自然科学基金项目(No.2021MS03083)。
