摘要
目的探讨外周血单个核细胞长链非编码RNA TMEVPG1、NRON的表达水平与精神分裂症患者症状和认知功能的关系。方法用荧光定量PCR法检测68例首发未服药精神分裂症患者(精神分裂症组)和50例健康人(对照组)外周血单个核细胞TMEVPG1、NRON的表达水平。采用阳性与阴性症状量表(PANSS)评价症状严重程度;MATRICS公认认知成套测验(MCCB)评价认知功能。结果精神分裂症组TMEVPG1、NRON的相对表达量分别为(4.80±1.12)和(3.14±1.24),对照组分别为(2.06±0.89)和(1.67±0.73),组间比较差异均有统计学意义(P<0.01)。TMEVPG1、NRON诊断精神分裂症的受试者工作特征曲线下面积(AUC)分别为0.801和0.719,灵敏度分别为80.56%和72.95%,特异度分别为73.33%和81.10%,两者联合检测可将AUC提高至0.854。与对照组比较,精神分裂症组信息处理加工速度、注意警觉性、工作记忆、言语学习、视觉学习、推理问题解决和社会认知均较差(P<0.01)。TMEVPG1和NRON水平均与PANSS量表阴性症状评分和总分呈正相关(r分别为0.480和0.370,P<0.05)。TMEVPG1与精神分裂症患者信息处理加工速度和工作记忆呈负相关(r分别为-0.321和-0.402,P<0.05),NRON水平与注意警觉性呈负相关(r=-0.341,P<0.05)。结论外周血单个核细胞TMEVPG1、NRON的表达与精神分裂症患者的阴性症状及认知功能有关。
Objective To investigatethe relationship between the expression of TMEVPG1 and NRON in peripheral blood mononuclear cells and the symptoms and cognitive function of schizophrenia.Methods The expression of TMEVPG1 and NRON in peripheral blood mononuclear cells of 68 patients(schizophrenia group)and 50 healthy people(control group)were detected by fluorescent quantitative PCR.PANSS and MCCB were used to evaluate the severity of symptoms and MATRICS consensus cognitive battery(MCCB)was used to evaluate the cognitive function.Results The relative expression of TMEVPG1 and NRON were(4.80±1.12)and(3.14±1.24)in schizophrenia group,and(2.06±0.89)and(1.67±0.73)in control group,respectively.There was significant difference between the two groups(P<0.01).The area under the receiver operating characteristic curve(AUC)of TMEVPG1 and NRON in the diagnosis of schizophrenia were 0.801 and 0.719,respectively.The sensitivity was 80.56%and 72.95%,and the specificity was 73.33%and 81.10%,respectively.The AUC of the combined detection of TMEVPG1 and NRON was increased to 0.854.Compared with the control group,the speed of information processing,attention alertness,working memory,speech learning,visual learning,reasoning problem-solving and social cognition of schizophrenia group were worse(P<0.05).TMEVPG1 and NRON were positively correlated with PANSS negative symptom score and total score(r=0.480 and 0.370,respectively,P<0.05).TMEVPG1 was negatively correlated with information processing speed and working memory in schizophrenics(r=-0.321 and-0.402,respectively,P<0.05).And NRON was negatively correlated with attention alertness(r=-0.341,P<0.05).Conclusion The expression of TMEVPG1 and NRON in peripheral blood mononuclear cells is related to the negative symptoms and cognitive function of patients with schizophrenia.
作者
祁娜
白田妹
徐天朝
QI Na;BAI Tian-mei;XU Tian-chao(Department of Psychology,General Hospital of Northen Theater Command,Shenyang 110016,Liaoning,China;Department of Obstetrics and Gynecology,General Hospital of Northen Theater Command,Shenyang 110016,Liaoning,China)
出处
《东南国防医药》
2022年第2期152-156,共5页
Military Medical Journal of Southeast China
关键词
精神分裂症
外周血
长链非编码RNA
认知功能
schizophrenia
peripheral blood
long-chain non coding RNA
cognitive function
