摘要
目的 了解上海市松江区腹泻患者副溶血弧菌的毒力基因、耐药性和分子分型等,为预防食源性疾病提供科学依据。方法 参照WS 271-2007进行副溶血弧菌的检测和分离,并应用微量肉汤法进行药物敏感性试验,采用PCR方法检测毒力基因,并对菌株进行脉冲场凝胶电泳(PFGE)分子分型。结果 2016—2020年采集腹泻患者肛拭子标本2 053份,共检出副溶血弧菌155株,阳性率为7.55%,其中夏秋季阳性率较高(11.80%),与冬春季(0.13%)的差异有统计学意义(χ^(2)=92.725,P<0.01)。2019年阳性率最高(10.55%),与2016和2020年相比差异有统计学意义(χ^(2)=8.890, P<0.05),但5年阳性率差异无统计学意义(χ^(2)=4.602, P>0.05)。155株副溶血弧菌均携带毒力基因tlh,其中87.74%携带tdh,2.58%携带trh,8.39%不携带tdh和trh,三者差异有统计学意义(χ^(2)=317.890,P<0.01)。副溶血弧菌对多种抗生素敏感,对头孢唑林的耐药率最高(56.25%),其次为氨苄西林和环丙沙星,且存在多重耐药。PFGE分子分型显示,155株副溶血弧菌分为7个簇,共80种型别,部分菌株100%同源,为优势菌株。结论 上海市松江区腹泻患者副溶血弧菌检出率较高,大部分携带毒力基因tdh,且对头孢唑林耐药严重,5年内优势菌株存在聚集性,须引起重视。
Objective To understand the virulence genes, antibiotics resistance and molecular typing of Vibrio parahaemolyticus isolated from diarrhea patients in Songjiang district, Shanghai, and provide scientific basis for the better prevention of foodborne diseases. Methods V. parahaemolyticus detection was conducted according to WS271-2007, and the isolated strains were used for antimicrobial susceptibility testing by broth microdilution method, virulence genes detection by PCR and molecular typing by pulsed-field gel electrophoresis(PFGE). Results From 2016 to 2020, a total of 2 053 anal swabs from patients with diarrhea were collected, and 155 strains of V. parahaemolyticus were isolated. The average detection rate was 7.55%. The detection rate was statistically higher in summer and autumn(11.80%) than in winter and spring(0.13%)(χ^(2)=92.725, P<0.01). The detection rate in 2019 was higher(10.55%) than those in other years, and there was a significant difference compared with 2016 and 2020(χ^(2)=8.890, P<0.05), but there were no significant differences in detection rate among five years(χ^(2)=4.602, P>0.05). All the 155 V. parahaemolyticus strains carried virulence gene tlh, 87.74% carried tdh, 2.58% carried trh, and 8.39% carried no tdh and trh. The differences were significant(χ^(2)=317.890, P<0.01).V. parahaemolyticus was sensitive to various antibiotics, and the resistance rate to cefazolin was highest(56.25%). Some strains were resistant to ampicillin and ciprofloxacin, and 3 strains were multi-drug resistant. PFGE molecular typing results showed that there were 7 clusters of V. parahaemolyticus, with 80 PFGE types. Some strains isolated from 2016 to 2020 shared100% homology, and they were predominant strains. Conclusion The detection rate of V. parahaemolyticus was high in patients with diarrhea in Songjiang district of Shanghai. Most strains detected carried virulence gene tdh, and they were highly resistant to cefazolin. The predominant strains clustered within five years, to which attention must be paid.
作者
俞佳莉
乔雪飞
盛峰松
钱懿青
吴健灏
吴佳瑾
李欣
Yu Jiali;Qiao Xuefei;Sheng Fengsong;Qian Yiqing;Wu Jianhao;Wu Jiajin;Li Xin(Songjiang District Center for Disease Control and Prevention,Shanghai 201620,China)
出处
《疾病监测》
CAS
CSCD
北大核心
2022年第2期245-250,共6页
Disease Surveillance
基金
上海市卫生健康委员会青年课题(No.20184Y0059)
上海市卫生健康委员会面上课题(No.201740147)。
关键词
副溶血弧菌
药物敏感性
毒力基因
脉冲场凝胶电泳
Vibrio parahaemolyticus
Virulence gene
Antimicrobial susceptibility
Pulsed-field gel electrophoresis
