摘要
为了解林窗位置对香椿细根分解及土壤真菌群落多样性的影响,以川中丘陵区30a生柏木人工林为研究对象,采用Illumina Miseq高通量测序技术研究200 m;人工林窗中心位置(GC)、边缘位置(GB)、郁闭林(CC)对香椿细根2a自然分解后土壤真菌群落结构及多样性的影响。结果表明,林窗内不同位置之间微环境差异显著(P<0.05),土壤理化性质对林窗位置变化的响应较细根养分元素更积极,GB位置细根分解速率显著高于GC和CC。土壤真菌门水平在3个林窗位置间无显著差异,其中子囊菌门、担子菌门是优势类群;腐生营养型真菌在3个林窗位置上具有显著差异,散囊菌目、粪壳菌目、肉座菌目、刺盾炱目、伞菌目是3个位置上相对丰度值>1%的优势类群,刺盾炱目、伞菌目相对丰度在GB和CC之间有显著差异(P<0.05);青霉属和曲霉属是真菌在属水平上的优势类群,其相对丰度在3个位置间有极显著差异(P<0.01)。林窗位置未对真菌群落α多样性产生显著影响,但群落β多样性对位置变化的响应显著。GB位置群落β多样性显著高于GC和CC(P<0.05),共有种数量最多,GC位置特有种数量最多。冗余分析发现较多环境因子对真菌群落的影响显著(P<0.01),Mantel test(和partial Mantel test)分析结果表明土壤pH值、土壤含水量、土壤温度、土壤速效磷、土壤全氮是真菌群落多样性的重要影响因子。综上所述,林窗内位置对土壤真菌类群构成和群落β多样性具有差异性影响,在柏木人工林内实施林窗式干扰有助于调节细根分解等地下生态进程。
The effect of gap location heterogeneity on decomposition of plant fine roots and soil fungal community diversity was investigated in a forest with Cupressus funebris plantation for 30 years, in the hilly region in central Sichuan Province. In an area with gap size 200 m;, the structure and diversity of soil fungal community associated with 2 a natural decomposition of fine root of Toona sinensis were investigated separately on three different locations: gap center(GC), gap boarder(GB) and closed canopy(CC), by using Illumina MiSeq high-throughput sequencing technology. The results showed that there were significant differences in microenvironment across different gap locations(P<0.05). The response of soil physical and chemical properties to the gap position was more sensitive than that of fine root nutrient elements, and the decomposition rate of fine roots at GB position was significantly higher than that of GC and CC. There was no significant difference in soil fungal community at phylum level. Ascomycetes and Basidiomycetes were dominant groups across different gap locations. Base on FUNGuild results, the trophic modes of saprotrophs showed significant differences across different gap locations, the classes of Eurotiales, Hypocreales, Sordariales, Chaetothyriales and Agaricales were dominant with the relative abundances>1% across the three locations. The relative abundances of Agaricales and Chaetothyriales were significantly different between GB and CC(P<0.05). The genera of Penicillium, Aspergillus, Chaetomium and Staphylotrichum were the dominant taxa, and there were significant differences in their relative abundances across the three locations(P<0.01). The α diversities of the fungal communities were not significantly affected by the gap locations, but their β diversities were more responsive to different gap locations. The β diversity at GB was significantly higher than those at GC and CC(P<0.05), with the largest number of shared species at GB and the largest number of endemic species at GC site. Redundancy analysis revealed significant effects of the environmental factors on the fungal community(P<0.01). Partial Mantel test showed that the factors such as pH, water content(SWC), temperature(ST), available P(SNP) and total N(STN) in soil were important factors influencing fungal community diversity. In conclusion, forest gap locations significantly affected the composition and diversity of soil fungal community. The findings were helpful to regulate the underground ecological processes such as fine root decomposition in cypress plantation forests.
作者
李德会
李相君
吴庆贵
尹海峰
李贤伟
LI Dehui;LI Xiangjun;WU Qinggui;YIN Haifeng;LI Xianwei(Ecological Security and Protection Key Laboratory of Sichuan Province,Mianyang Normal University,Mianyang 621000,China;Collage of Forestry,Sichuan Agricultural University,Chengdu 611030,China)
出处
《生态学报》
CAS
CSCD
北大核心
2022年第7期2784-2797,共14页
Acta Ecologica Sinica
基金
国家重点研发计划项目(2017YFD0600302-05)
国家自然科学基金项目(32071747)
绵阳师范学院生态安全与保护四川省重点实验室开放基金项目(ESP-2104)
绵阳师范学院高层次人才科研启动项目(QD2020A17)。
关键词
柏木人工林
林窗位置
细根分解
高通量测序
真菌群落多样性
cypress plantation forest
gap locations
fine root decomposition
MiSeq high-throughput sequencing
fungal community diversity
