摘要
目的探讨关于黄精对大鼠肝脏纤维化的干预效果及对肝脏的保护作用。方法研究对象来源选取Wistar大鼠,对实验动物血样及组织进行分析;样本总数45只;分为正常对照组、肝纤维化模型组以及黄精治疗干预组,每组15只。测定大鼠肝外周血TG F-β水平,使用ELISA法完成;使用免疫组化法对肝组织标本中的TGF-β1、Smad3、Smad7蛋白表达水平进行测定;并对组织标本的3种蛋白的mRNA表达同时进行测定,使用RT-PCR方法完成,且观察病理变化。SPSS23.0行数据分析,方法包括t检验、F检验和卡方检验等。结果实验动物外周血和组织中TGF-β1表达均可见对照组水平最低(0.72±0.21),肝纤维化造模后的表达水平最高,而干预组在使用黄精干预后可见其表达水平减低(治疗后为1.65±0.69),但仍高于对照组水平;组织中TGF-β1表达阳性率以及mRNA表达可见相似趋势;组织Smad3蛋白及mRNA表达量均可见对照组水平最低(0.21±0.03),肝纤维化造模后的表达水平最高(7.54±0.25),而干预组在使用黄精干预后可见其表达水平减低(治疗后为4.53±0.17),但仍高于对照组水平;组织Smad7蛋白及mRNA表达量均可见对照组水平最低,肝纤维化造模后的表达水平增高,而干预组在使用黄精干预后表达水平最高;经黄精干预后的大鼠,纤维化病变程度有所减轻。结论研究中可见黄精的干预对于改善大鼠肝纤维化病变充分有效,其作用机制与TGF-β1/Smad信号通路密切关联,可见病理变化中TGF-β1及Smad7蛋白和基因表达增高,干预后有所减低。
Objective To discusses the intervention effect of polygonatum on liver fibrosis in rats and its protective effect on liver. Methods Wistar rats were selected as the source of the research subjects,and the blood samples and tissues of experimental animals were analyzed;the total number of samples was 45;they were divided into normal control group,liver fibrosis model group and Huangjing treatment intervention group,each with 15 rats. The study was used to determine the level of TG F-β in the peripheral blood of rats by ELISA;the expression levels of TGF-β1,Smad3,and Smad7 in liver tissue specimens were determined by immunohistochemistry;and the three proteins in tissue specimens The expression of mRNA was measured at the same time,using the RT-PCR method to complete;and observe the pathological changes. SPSS 23.0 line data analysis,methods include t test,F test and Chi-square test. Results The expression of TGF-β1 in the peripheral blood and tissues of experimental animals showed the lowest level in the control group(0.72±0.21),and the highest expression level after hepatic fibrosis modelling,while the intervention group showed that its expression level decreased after the use of Polygonatum(After treatment:1.65±0.69),but still higher than the control group. The positive rate of TGF-β1 expression and mRNA expression in tissues showed similar trends. The expression of Smad3 protein and m RNA in tissues showed the lowest level in the control group(0.21±0.03),and the highest expression level after hepatic fibrosis modelling(7.54±0.25),while the expression level of the intervention group was reduced after the intervention of Polygonatum(after treatment:4.53±0.17),but still higher than the control group. The expression levels of Smad7 protein and mRNA in tissues showed that the control group had the lowest level,the expression level of liver fibrosis model was increased,and the intervention group had the highest expression level after the intervention of Polygonatum. After the intervention of Polygonatum,the degree of fibrotic lesions was reduced. Conclusion The research shows that the intervention of Polygonatum is fully effective in improving liver fibrosis in rats,and its mechanism of action is closely related to the TGF-β1/Smad signaling pathway. It can be seen that the expression of TGF-β1 and Smad7 proteins and genes in pathological changes increases. After intervention reduced.
作者
符蓉
曹斌
FU Rong;CAO Bin(Department of Pathology,Hai′an Hospital,Nantong University,Nan-tong,Jiangsu Province 226600,China)
出处
《解剖学研究》
CAS
2022年第1期7-11,共5页
Anatomy Research
