摘要
目的探讨妊娠期糖尿病患者血清微小核糖核酸-15a(miR-15a)表达水平变化情况及其与母婴不良结局的关系。方法选取妊娠期糖尿病患者143例为实验组,另选取同期孕检健康的124例正常孕妇为对照组,采用实时荧光定量逆转录聚合酶链反应(qRT-PCR)检测血清miR-15a表达水平。随访至分娩,观察两组母婴不良结局发生情况,并采用多因素Logistic回归性分析法分析血清miR-15a表达水平与妊娠期糖尿病患者母婴不良结局的关系。结果实验组血清miR-15a表达水平高于对照组,差异有统计学意义(P<0.05);实验组流产、胎膜早破、羊水过多、产后出血、产后感染、早产儿、巨大儿、胎儿窘迫、新生儿窒息、新生儿呼吸窘迫综合征、新生儿低血糖发生率及母体、围生儿不良结局总发生率均高于对照组(P<0.05),实验组死胎发生率与对照组比较差异无统计学意义(P>0.05);母体结局不良组与围生产儿结局不良组的年龄>35岁、孕前体质量指数≥24.0 kg/m^(2)、不良孕产史、血糖控制不良占比及血清miR-15a表达水平均高于母体结局良好组、围生产儿结局良好组(P<0.05),经多因素Logistic回归性分析显示,以上各指标均是妊娠期糖尿病患者母婴不良结局的危险因素(P<0.05)。结论妊娠期糖尿病患者血清miR-15a表达水平异常升高,可增加母婴不良结局发生风险,与母婴不良结局的发生关系密切。患者年龄、孕前体质量指数、不良孕产史、血糖控制情况等亦对妊娠期糖尿病患者母婴不良结局的发生具有一定的影响。
Objective To investigate the change of serum microRNA-15a(miR-15a)expression level in patients with gestational diabetes mellitus and its relationship with maternal and infant adverse outcomes.Methods 143 cases of gestational diabetes mellitus patients were selected as the study group,and 124 healthy pregnant women were selected as the control group,and the expression levels of serum miR-15a of the two groups were detected by real time fluorescent quantitative reverse transcription polymerase chain reaction(qRT-PCR).Follow up to delivery,the adverse maternal and infant outcomes of the two groups were observed,and the relationship between the expression level of serum miR-15a and the adverse outcomes of maternal and infant in gestational diabetes mellitus was analyzed by multivariate Logistic regression analysis method.Results The expression level of serum miR-15a in the study group was higher than that in the control group,and the difference was statistically significant(P<0.05).The incidence rates of abortion,premature rupture of membranes,polyhydramnios,postpartum hemorrhage,postpartum infection,premature infants,macrosomia,fetal distress,neonatal asphyxia,neonatal respiratory distress syndrome and neonatal hypoglycemia and the total incidence rates of maternal and perinatal adverse outcomes in the study group were higher than those in the control group(P<0.05),but there was no significant difference in the incidence rate of fetal death between the study group and the control group(P>0.05).The proportions of age>35 years old,pre-pregnancy body mass index≥24.0 kg/m^(2),history of adverse pregnancy and childbirth and poor blood glucose control and the expression level of serum miR-15a in poor maternal outcome group and poor perinatal outcome group were higher than those in good maternal outcome group and good perinatal outcome group(P<0.05),and multivariate Logistic regression analysis showed that the above indicators were the risk factors of adverse maternal and infant outcomes in patients with gestational diabetes mellitus(P<0.05).Conclusion The expression level of serum miR-15a in gestational diabetes mellitus patients abnormally increases,and it can increase the risk of adverse maternal and infant outcomes,which is closely related to the occurrence of adverse maternal and infant outcomes.Besides,the age,pre-pregnancy body mass index,adverse pregnancy history,blood glucose control also have a certain impacts on the occurrence of adverse maternal and infant outcomes in patients with gestational diabetes mellitus.
作者
马玉着
陈素玉
刘昱婕
Ma Yuzhuo;Chen Suyu;Liu Yujie(Dept of Obstetrics and Gynecology,The First Affiliated Hospital of USTC,Hefei 230002)
出处
《安徽医科大学学报》
CAS
北大核心
2022年第4期650-654,共5页
Acta Universitatis Medicinalis Anhui
基金
安徽省博士后研究人员科研活动经费资助项目(编号:2019B373)。
关键词
妊娠期糖尿病
微小核糖核酸-15a
母婴结局
关系
gestational diabetes mellitus
microRNA-15a
maternal and infant outcomes
relationship
