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大豆疫霉生防菌解淀粉芽孢杆菌JDF3的鉴定 被引量:7

Identification of Bacillus amyloliquefaciens JDF3 as a biocontrol agent against Phytophthora sojae
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摘要 分离获得1株细菌JDF3,它能有效地抑制大豆疫霉菌丝的生长和孢子囊的形成。该菌株对细极链格孢、小麦全蚀病菌和西瓜炭疽病菌的抑制率高于70%。盆栽结果显示细菌JDF3对大豆疫病的防治率为70.7%。菌株形态特征、18项生理生化和gyrB基因测序结果鉴定菌株JDF3为解淀粉芽孢杆菌。 A strain JDF3 with a good control effect against Phytophthora sojae was isolated,which could inhibit the growth of P.sojae and the formation of sporangia.It had a broad spectrum of inhibition against Alternaria tenuissima,Gaeumannomyces gramini and Colletotrichum orbiculare with inhibition rate over 70%.The potted results showed that the control rate of soybean Phytophthora root and stem rot was 70.7%.The strain JDF3 was identified as Bacillus amyloliquefaciens with morphological characteristics,the results of 18 physiological and biochemical,and gyrB gene sequencing.
作者 刘冬 胡九龙 李坤缘 李萍 高智谋 LIU Dong;HU Jiulong;LI Kunyuan;LI Ping;GAO Zhimou(School of Plant Protection,Anhui Agricultural University,Hefei 230036;School of Life Sciences,Anhui Agricultural University,Hefei 230036;Department of Horticulture and Landscape,Anqing Vocational and Technical College,Anqing 246003)
出处 《安徽农业大学学报》 CAS CSCD 2022年第1期1-5,共5页 Journal of Anhui Agricultural University
基金 国家自然科学基金(31671977) 安徽省高校自然科学研究重大项目(KJ2021ZD0164) 安徽省高校优秀青年人才支持计划重点项目(gxyqZD2018120) 刘冬技术技能型大师工作室(2019dsgzs19)共同资助。
关键词 大豆疫霉 解淀粉芽孢杆菌 鉴定 生物防治 Phytophthora sojae Bacillus amyloliquefaciens identification biocontrol
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