摘要
目的探讨蛋白基因产物9.5(PGP 9.5)在子宫内膜样癌中的表达及其与临床病理特征的关系。方法选取2017年1月至2020年12月赣州市妇幼保健院收治的91例子宫内膜样癌患者以及20例健康者作为研究对象,将其子宫内膜样癌组织标本以及正常子宫内膜组织标本进行制作、切片,通过免疫组化实验对PGP 9.5进行染色并判读结果,对比正常子宫内膜组织和子宫内膜样癌组织中PGP 9.5表达情况,根据表达高低将子宫内膜样癌标本分为高表达组和低表达组,比较两组患者的年龄、肿瘤直径、组织学分级、肌层浸润、宫颈管累及、淋巴结转移、脉管累及、附件累及、国际妇产科协会(FIGO)分期等临床病理特征。结果免疫组化实验结果显示,正常子宫内膜组织中PGP 9.5的阳性表达率低于子宫内膜样癌组织,差异有统计学意义(P<0.05)。两组患者年龄比较,差异无统计学意义(P>0.05),两组患者肿瘤直径、组织学分级、肌层浸润、宫颈管累及、淋巴结转移、脉管累及、附件累及、FIGO分期比较,差异有统计学意义(P<0.05)。结论PGP 9.5在子宫内膜样癌组织、正常子宫内膜组织中的表达水平存在差异,说明该蛋白基因产物在子宫内膜组织由正常转变为子宫内膜样癌的发生的过程中具有重要调控作用。因此可将其作为子宫内膜样癌预后的有效预测指标,为临床治疗子宫内膜样癌提供新思路、新方法,可创造一定的社会效益。
Objective To investigate the expression of protein gene product 9.5(PGP 9.5)in endometrioid carcinoma and its relationship with clinicopathological characteristics.Methods From January 2017 to December 2020,91 patients with endometrioid cancer and 20 healthy patients who were admitted to Ganzhou Maternity and Child Health Hospital were selected as the research objects,and their tissue specimens of endometrioid cancer and normal endometrium were collected.Tissue specimens were prepared and sectioned.PGP 9.5 was stained by immunohistochemical experiments and the results were interpreted.The expression of PGP 9.5 in normal endometrial tissue and endometrioid carcinoma tissue was compared.The cancer specimens were divided into high expression group and low expression group.The clinicopathological characteristics such as age,tumor diameter,histological grade,muscle invasion,cervical canal involvement,lymph node metastasis,vascular involvement,adnexal involvement,Federation International of Gynecology and Obstetrics(FIGO)staging,etc.were compared between the two groups.Results The results of immunohistochemical experiments showed that the positive expression rate of PGP 9.5 in normal endometrial tissues was lower than that in endometrioid carcinoma tissues,and the difference was statistically significant(P<0.05).The age of the two groups of patients were compared,and the difference was not statistically significant(P>0.05).The tumor diameter,histological grade,myometrial infiltration,cervical canal involvement,lymph node metastasis,vascular involvement,adnexal involvement,FIGO staging were compared between the two groups,and the differences were statistically significant(P<0.05).Conclusion The expression level of PGP 9.5 in endometrioid carcinoma tissues and normal endometrial tissues is different,indicating that the protein gene product has a role in the process of endometrial tissue transforming from normal to endometrioid carcinoma.Important regulatory role.Therefore,it can be used as an effective predictor of the prognosis of endometrioid carcinoma,providing new ideas and new methods for clinical treatment of endometrioid carcinoma,which can create certain social benefits.
作者
曾繁晟
刘兰兰
杨晶珍
ZENG Fancheng;LIU Lanlan;YANG Jingzhen(Department of Pathology,Ganzhou Maternal And Child Health Hospital,Jiangxi Province,Ganzhou 341000,China;Department of Gynecology,Ganzhou Maternal And Child Health Hospital,Jiangxi Province,Ganzhou 341000,China)
出处
《中国当代医药》
CAS
2022年第8期47-50,F0003,共5页
China Modern Medicine
基金
江西省赣州市卫生健康委员会科研计划项目(2020-2-61)。
关键词
蛋白基因产物9.5
子宫内膜样癌
免疫组化
基因表达
临床病理
Protein gene product 9.5
Endometrioid carcinoma
Immunohistochemistry was used
Gene expression
Clinical pathology
