摘要
目的分析四个哮喘易感基因单核苷酸多态性(Single nucleotide polymorphism,SNPs)与不同体质毛细支气管炎后喘息的相关性,为毛细支气管炎预后的判断和干预提供依据。方法纳入我科毛细支气管炎住院患儿132例,分为特应性体质组(atopic group,A组)63人和非特应性体质组(non-atopic group,N组)69人。入院常规检测血常规、呼出气一氧化氮(fractional exhaled nitric oxide,FeNO)、四个哮喘易感基因SNPs:IL13 A2044G、IL4-590C>T、ADRβ2 R16G和FcεR1βE237G,并进行为期1年随访,随访指标为出院后喘息发作情况,比较两组间上述指标的差异。结果与N组相比,A组患儿外周血嗜酸性粒细胞比例、出院1年内喘息再次发作人数及喘息频次显著升高(P<0.01),A组的哮喘易感基因SNPs ADRβ2 R16G AA基因型频率显著高于N组,而GG基因型频率低于N组,两组间存在显著统计学差异(P<0.05),另外三个哮喘易感基因多态性位点IL13 A2044G、IL4-590C>T、FcεR1βE237G及FeNO水平两组间比较无统计学差异(P>0.05)。结论哮喘易感基因SNPs ADRβ2 R16G AA基因型高频率及GG基因型低频率,对预警毛细支气管炎后喘息及哮喘发作风险具有一定价值,可作为早期甄别哮喘高危儿的指标。
Objective To analyze the correlation between single nucleotide polymorphisms(SNPs)of four asthma susceptibility genes and wheezing after bronchiolitis of different constitutions,and to provide a basis for prognostic judgment and intervention of bronchiolitis.Methods A total of 132 children with bronchiolitisin treated in our department were included,and they were divided into 63 atopic group(groupA)and 69 non-atopic group(group N).Blood routine,fractional exhaled nitric oxide(FeNO),four single nucleotide polymorphisms sites of asthma susceptibility genes:IL13A2044G,IL4-590C>T,ADRβ2 R16G,and FcεR1βE237G were detected.The patients were followed up for one year,and the follow-up index was the onset of wheezing after discharge,and the differences in the above indicators between the two groups were compared.Results Compared with group N,the proportion of peripheral blood eosinophils,the number of recurrent wheezing and the frequency of wheezing within 1 year after discharge in group A were significantly increased(P<0.01).The frequency of ADRβ2 R16G AA genotype of asthma susceptibility gene SNPs in group A was significantly higher than that in group N,while the frequency of GG genotype was lower than that in group N,and there was a statistically significant difference between the two groups(P<0.05).The other three asthma susceptibility gene polymorphisms IL13 A2044G,IL4-590C>T,FcεR1βE237G and FeNO levels had no statistical difference between the two groups(P>0.05).Conclusions The high frequency of AA homozygote and low frequency of GG homozygote of SNPs ADRβ2 R16G have potential value in early warning of wheezing and asthma attack risk after bronchiolitis and can be used as an indicator for identification of high-risk children with asthma.
作者
钱前
张玉红
王宜芬
李伟
刘丽
于艳艳
QIAN Qian;ZHANG Yu-hong;WANG Yi-fen;LI Wei;LIU Li;YU Yan-yan(Department of Pediatric Respiration,Lianyungang Maternal and Child Health Hospital,Lianyungang,Jiang Su 222000,China)
出处
《临床肺科杂志》
2022年第5期676-680,共5页
Journal of Clinical Pulmonary Medicine
基金
连云港市卫生计生青年科技项目(No.QN1810)。
关键词
哮喘易感基因
毛细支气管炎
特应性体质
喘息
asthma susceptibility gene
bronchiolitis
atopic constitution
wheezing
