摘要
为探究PD-Anammox耦合工艺快速启动过程中基质波动对微生物菌群结构及氮代谢功能基因的响应机制,通过改变进水的基质比例,联合宏基因组学和宏转录组学分析技术,深度解析耦合工艺的脱氮效能、微生物群落结构及氮代谢功能基因的变化规律。结果表明,PD-Anammox工艺的总氮去除率在进水氮源基质改变初期大幅下降,长期运行后其贡献率高达97.1%。变形菌门的相对丰度与进水中硝酸盐氮的含量呈正相关,而浮霉菌门的相对丰度与碳源浓度呈负相关。氮源瞬时冲击时,厌氧氨氧化功能基因hdh/hzo和hzsA/B/C、硝酸盐还原基因napA/B/C和nar B/C、亚硝酸盐还原基因nirS等表达量均显著下降,1 d后厌氧氨氧化和反硝化功能基因表达量显著回升。长期运行后,PD-Anammox耦合系统恢复脱氮效能,与冲击前的系统相比,hdh/hzo和hzsA/B/C基因表达量降低,而nos Z基因表达量升高。
In order to investigate response mechanism of substrate fluctuations on microbial community structure and nitrogen metabolism functional genes during the rapid start-up process of partial denitrification and anammox coupling system(PD-Anammox),the nitrogen removal efficiency,microbial community structure and nitrogen metabolism functional genes of the coupled process were analyzed by changing the nitrogen substrate ratio and combining metagenomic and metatranscriptomic techniques.The results showed that the total nitrogen removal rate of PDAnammox process decreased significantly at the initial stage of the influent nitrogen source change,but its contribution was as high as 97.1%after long-term operation.There was a positive correlation between relative abundance of Proteobacteria and content of nitrate nitrogen in influent,while relative abundance of Planctomycetes was negatively correlated with the concentration of carbon source.The functional genes of anaerobic ammonia oxidation(hdh/hzo and hzsA/B/C),nitrate reduction genes(napA/B/C and narB/C),nitrite reduction genes(nirS)were significantly down-expressed after instantaneous nitrogen source shock.The expression levels of functional genes in anammox and denitrification increased significantly after the first day.After long-term operation,the PD-Anammox system recovered its nitrogen removal efficiency.Compared with the coupled system before nitrogen source shock,the expression levels of hdh/hzo and hzsA/B/C decreased,while the expression levels of nosZ increased.
作者
刘宁
何阳
王德朋
杨庆
邱晨晨
罗琦
黄开龙
张徐祥
LIU Ning;HE Yang;WANG Depeng;YANG Qing;QIU Chenchen;LUO Qi;HUANG Kailong;ZHANG Xuxiang(Jinling College,Nanjing University,Nanjing 210032,China;State Key Laboratory of Pollution Control and Resource Reuse,School of Environment,Nanjing University,Nanjing 210023,China;Nanjing Jiangdao Institute of Environmental Research Co.,Ltd.,Nanjing 210019,China)
出处
《工业水处理》
CAS
CSCD
北大核心
2022年第4期57-64,共8页
Industrial Water Treatment
基金
国家自然科学基金项目(52100082)
江苏省重点研发计划(社会发展)项目(BE2019635)
污染控制与资源化研究国家重点实验室开放基金项目(PCRRF19004,PCRRF20018)
江苏高校青蓝工程中青年学术带头人(苏教师[2017]15号)
2020年度南京大学金陵学院校企共建课题“水处理工程设计”(0010522014)。
关键词
短程反硝化-厌氧氨氧化
基质波动
快速启动
氮代谢功能基因
微生物
partial denitrification and anammox
substrate fluctuations
rapid start-up process
nitrogen metabolic genes
microorganism
