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高效液相色谱法测定蒙药绍沙-7丸中没食子酸含量 被引量:3

Determinition of gallic acid in Mongolian medicine Shaosha-7 Pills by HPLC
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摘要 目的:建立检测蒙药绍沙-7丸中没食子酸含量的高效液相色谱法,为其质量标准提供依据。方法:色谱柱为Capcell Pak C;(4.6 mm×250 mm,5μm),流动相为0.1%磷酸(0.1%三乙胺)水溶液-3%乙腈溶液(97∶3),流速为1.0 mL/min,检测器波长271 nm,柱温为30℃,进样量为10μL。结果:没食子酸在61.17~917.55 ng范围内呈良好的线性关系(R;=1),该方法精密度(RSD为0.77%)、准确度(平均回收率为105.9%,RSD为1.09%)、稳定性(RSD为0.99%)良好。用以上方法测得2个批次绍沙-7丸中没食子酸平均含量分别为0.1789、0.1599 mg/g。结论:该方法简单、准确、灵敏度高、稳定性好,可用于蒙药绍沙-7丸中没食子酸的含量测定。 Objective: To establish the method for the determination of gallic acid in Mongolian medicine Shaosha-7 Pills by HPLC, and provide basis for its quality standard. Methods: The chromatography column was Capcell Pak C;(4.6 mm×250 mm, 5 μm);the mabile phase was 0.1% phosphoricacid (containing 0.1% triethylamine) aqueous solution-3% acetonitrile (97∶3);the flow rate was 1.0 mL/min;the detector wavelength was 271 nm, the column temperature was 30 ℃, and injection volume was 10 μL.Results: Gallic acid had a good linear relationship in the range of 61.17~917.55 ng (R2=1). This method showed good precision (RSD was 0.77%), accuracy (average recovery was 105.9%, RSD was 1.09%) and stability (RSD was 0.99%). The content of gallic acid in two different production batches of Shaosha-7 Pills was 0.1789 and 0.1599 mg/g. Conclusion: This method is simple, accurate and stable, which can be used for the determinition of gallic acid in Mongolian medicine Shaosha-7 Pills.
作者 杭呼格吉乐 王青亮 银苹 高娃 张文 那松巴乙拉 锡林其其格 斯琴 都格尔 乌汉其木格 Hanghugejile;WANG Qing-liang;YIN Ping;Gaowa;ZHANG Wen;Nasongbayila;Xilinqiqige;Siqin;Dugeer;Wuhanqimuge(Inner Mongolia International Mongolian Hospital,Hohhot 010065,China;Inner Mongolia Medical University,Hohhot 010010,China)
出处 《中华中医药杂志》 CAS CSCD 北大核心 2022年第3期1710-1713,共4页 China Journal of Traditional Chinese Medicine and Pharmacy
基金 内蒙古自治区“草原英才”工程青年创新人才一层次资助基金项目(No.Q2017045) 内蒙古自治区第九批“草原英才”工程蒙药新药研发及产业化创新人才团队滚动支持项目(No.NEI ZU TONG ZI[2018]29) 内蒙古自治区科技计划项目(No.2021GG0126)。
关键词 高效液相色谱法 蒙药绍沙-7丸 没食子酸 含量测定 HPLC Mongolian medicine Shaosha-7 Pills Gallic acid Assay
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