摘要
[目的]优化壳寡糖纳米硒的合成工艺。[方法]采用单因素探究壳寡糖与亚硒酸钠的比例、乙酸质量浓度和反应时间对纳米粒子粒径、PDI和电位的影响,并以PDI为主要判断依据,电位为次要判断依据,进行正交试验,优化反应的条件。[结果]最优工艺条件为壳寡糖与亚硒酸钠的比例为16∶1,乙酸质量浓度为3%,反应时间为2.5 d,得到壳寡糖纳米硒的PDI为0.238,电位为50.36 mV。通过方差分析可知,反应条件对PDI影响由大到小依次为壳寡糖与亚硒酸钠的比例>反应时间>乙酸质量浓度,反应条件对电位影响由大到小依次为反应时间>壳寡糖与亚硒酸钠的比例>乙酸质量浓度。[结论]该研究优化了壳寡糖纳米硒的合成条件,为壳寡糖纳米硒的进一步开发利用提供了支持。
[Objective]To optimize the synthesis process of chitooligosaccharides-selenium nanoparticles.[Method]The single factor was used to explore the influence of the ratio of chitooligosaccharide to sodium selenite,the mass concentration of acetic acid and the reaction time on the particle size,PDI and potential of nanoparticles.Using PDI as the main judgment basis and potential as the secondary judgment basis,an orthogonal experiment was carried out to optimize the reaction conditions.[Result]The best process conditions for synthesizing chitooligosaccharides-selenium nanoparticles were the ratio of chitooligosaccharides to sodium selenite 16∶1,3%acetic acid mass concentration,and the reaction time 2.5 days.Under this condition,the PDI of the chitooligosaccharides-selenium nanoparticles was 0.238 and the potential was 50.36 mV.The variance analysis of the experimental factors showed that the effect of reaction conditions on PDI was in the order of the ratio of chitooligosaccharide to sodium selenite>reaction time>acetic acid mass concentration,and the effect of reaction conditions on potential is in the order of reaction time>the ratio of chitooligosaccharide to sodium selenite>acetic acid mass concentration.[Conclusion]The synthesis conditions of chitooligosaccharides-selenium nanoparticles are optimized,which provides support for the further development and utilization of chitooligosaccharides-selenium nanoparticles.
作者
叶大天
任祥雨
夏磊
林昌宇
肖云玉
闻正顺
YE Da-tian;REN Xiang-yu;XIA Lei(School of Food Science and Pharmaceutics,Zhejiang Ocean University,Zhoushan,Zhejiang 316022;Zhoushan Center of Agricultural Products Quality and Safety Supervision and Management,Zhoushan,Zhejiang 316021)
出处
《安徽农业科学》
CAS
2022年第8期156-159,共4页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目(81903693)
舟山市科技计划项目(2020C21018,2020C21014)
舟山市科技合作项目(2020C21047,2021C13044)。
关键词
壳寡糖
纳米硒
合成工艺
优化
正交试验
Chitooligosaccharides
Nano-selenium
Synthesis process
Optimization
Orthogonal experiment
