摘要
以桑蓓斯凤仙橘红、粉红2个品种的腋芽为外植体,开展茎尖脱毒与增殖诱导试验,探讨外植体选择、灭菌、腋芽诱导、增殖培养的最优条件,建立稳定的增殖体系。结果表明:采集1~2 cm大小的侧芽叶芽率最高;消毒9 min后剥离成0.3~0.4 mm大小茎尖,接种在MS+6-BA0.5/mg/L+NAA0.1mg/L的培养基中生长最佳;桑蓓斯凤仙组培苗瓶外生根,生根率达100%。
Axillary buds of two species of Impatiens Sangbei were used as explants to carry out stem tip detoxification and proliferation induction experiment to explore the optimal conditions for explant selection,sterilization,axillary bud induction,and proliferation culture,and to establish a stable proliferation system.The results showed that the highest rate of leaf buds was collected from side shoots of 1~2 cm in size.The stem tips were stripped into 0.3 to 0.4 mm in size after sterilization for 9 minutes and inoculated in medium of MS+6-BA0.5 mg/L+NAA0.1 mg/L for the best growth.The Impatiens Sangbei tissue culture seedlings took roots outside the bottle,and the rooting rate reached 100%.
作者
宋嘉玮
刘辉
张黎
Song Jiawei;Liu Hui;Zhang Li(School of Agriculture,Ningxia University,Yinchuan 750021,China)
出处
《农业科学研究》
2022年第1期79-83,共5页
Journal of Agricultural Sciences
基金
2019宁夏回族自治区重点研发计划项目(现代农业科技创新示范区专项2019BBF02011)“新优特异花卉引进筛选与配套栽培技术集成示范”。
关键词
桑蓓斯凤仙
茎尖脱毒
增殖
瓶外生根
Impatiens Sangbei
detoxification of stem tip
proliferation
rooting outside the bottle
