摘要
通过物理(紫外线诱变)和化学(氯化锂诱变)相结合的方法对209#己酸菌进行诱变,采用平板法对其进行单菌落分离。对分离得到的单菌种进行产酸性能测定,筛选出发生正向突变的菌种,并对其进行遗传性状稳定性测试。最终将筛选出的性状优良、稳定的菌种进行组合并应用到大生产中,从而达到提高己酸产量的目的。
Caproic acid-producing bacterial strain 209# was mutagenized by physical(ultraviolet) and chemical(lithium chloride)methods, and then isolated by plate method. The acid-producing capacity of the isolated strains was determined, and the strains with forward mutation were selected. The stability of the genetic traits of the selected strains was determined. Finally, the strains with excellent and stable properties were screened out and applied in mass production, which achieved the purpose of increasing caproic acid yield.
作者
屈慧
王海英
王慧
徐芳
屈宇
郝祯燕
QU Hui;WANG Haiying;WANG Hui;XU Fang;QU Yu;HAO Zhenyan(Technology Center of Hetao Distillery Group Co.Ltd.,Bayannur,Inner Mongolia 015400;Linhe District People’s Hospital,Bayannur,Inner Mongolia 015000;Linhe District Animal Disease Prevention and Control Center,Bayannur,Inner Mongolia 015000,China)
出处
《酿酒科技》
2022年第4期17-21,共5页
Liquor-Making Science & Technology
关键词
己酸菌诱变
单菌落分离
正突变菌
遗传性状
mutagenesis of caproic acid-producing bacteria
single colony separation
forward mutation
genetic traits
