摘要
背景与目的:HOTTIP和H19都是lncRNA(long non-coding RNA,lncRNA)中的一员,在肿瘤的发生、发展和迁移过程中发挥重要作用。本研究探讨HOTTIP rs2067087、H19 rs2839698和H19 rs2107425单核苷酸多态性(single nucleotide polymorphism,SNP)与不同级别子宫颈病变发病风险的关系。方法:收集2018年11月—2020年12月盐城市第一人民医院收治的345例子宫颈病变患者的外周静脉血及相关临床资料,其中子宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)220例,子宫颈癌(cervical cancer,CC)125例,选取同期住院的360例无子宫颈病变患者为对照。采用TaqMan实时荧光定量聚合酶链反应(real-time fluorescence quantitative polymerase chain reaction,RTFQ-PCR)方法对2组人群中的HOTTIP基因的SNP位点rs2067087和H19基因的SNP位点rs2839698、rs2107425进行基因分型,采用χ^(2)检验和非条件logistic回归分析3个SNP位点与不同级别子宫颈病变的相关性。结果:与对照组相比,HOTTIP rs2067087和H19 rs2839698在子宫颈病变患者中的基因型分布存在显著差异。在隐性模型中,携带HOTTIP rs2067087 GG基因型患者发生子宫颈病变的频率增加了52.2%(95%CI:1.021~2.269,P<0.05),分层分析发现,该位点GG基因型主要增加了CIN的发病风险(OR=1.730,95%CI:1.117~2.680,P<0.05);与CC+CT基因型相比,携带H19 rs2839698 TT基因型患者发生子宫颈癌的风险减少了70.8%(95%CI:0.087~0.976,P<0.05)。未发现H19 rs2107425位点与不同级别子宫颈病变相关(P>0.05)。结论:HOTTIP基因SNP位点rs2067087与不同级别子宫颈病变相关,其GG基因型可能是宫颈上皮内瘤变发生的危险因素;H19基因SNP位点rs2839698与子宫颈癌发病风险相关,其TT基因型可能是子宫颈癌发生的保护因素。
Background and purpose:Both HOTTIP and H19 are members of lncRNA(long non‑coding RNA).They have been found to play an important role in the occurrence,development and migration of tumors.To investigate the association between single nucleotide polymorphisms(SNP)of HOTTIP rs2067087、H19 rs2839698 and H19 rs2107425 and the risk of cervical lesions of different grades.Methods:The peripheral venous blood and related clinical data of 345 cases of cervical lesions were collected.The peripheral venous blood and related clinical data of 345 patients with cervical lesions were collected,including 220 cases of cervical intraepithelial neoplasia(CIN)and 125 cases of cervical cancer(CC).Three hundred and sixty patients without cervical lesions hospitalized in the same period were selected as the control.TaqMan real-time fluorescence quantitative polymerase chain reaction(RTFQ-PCR)was used to genotype the HOTTIP SNP rs2067087,H19 SNP rs2839698 and rs2107425 in the two groups,andχ2 test and unconditional logistic regression was used to analyze the association of the three SNP loci with cervical lesions of different grades.Results:Compared with the control group,there were significant differences in genotype distribution of HOTTIP rs2067087 and H19 rs2839698 in patients with cervical lesions.In the recessive model,the frequency of cervical lesions in patients with HOTTIP rs2067087 GG genotype increased by 52.2%(95%CI:1.021-2.269,P<0.05).Stratified analysis showed that GG genotype at this locus mainly increased the risk of CIN(or=1.730,95%CI:1.117-2.680,P<0.05);Stratified analysis showed that the GG genotype at this locus mainly increased the risk of CIN(OR=1.730,95%CI:1.117-2.680,P<0.05).Compared with CC+CT genotype,the risk of cervical cancer in patients with H19 rs2839698 TT genotype was reduced by 70.8%(95%CI:0.087-0.976,P<0.05).No correlation was found between H19 rs2107425 and cervical lesions of different grades(P>0.05).Conclusion:HOTTIP rs2067087 SNP is associated with different levels of cervical lesions,and the GG genotype may be a risk factor for cervical intraepithelial neoplasia;H19 rs2839698 SNP is associated with the risk of cervical cancer,and the TT genotype may be a protective factor for cervical cancer.
作者
王春桃
葛安兴
吴红雁
张学艳
杨圣
袁红香
程艳萍
冯延璐
陆欣苑
梁戈玉
WANG Chuntao;GE Anxing;WU Hongyan;ZHANG Xueyan;YANG Sheng;YUAN Hongxiang;CHENG Yanping;FENG Yanlu;LU Xinyuan;LIANG Geyu(Jiangsu Vocational College of Medicine,Yancheng 224001,Jiangsu Province,China;Key Laboratory of Environmental Medicine Engineering,Ministry of Education,School of Public Health,Southeast University,Nanjing 210009,Jiangsu Province,China;Department of Radiotherapy,Yancheng No.1 People's Hospital,Yancheng 224001,Jiangsu Province,China)
出处
《中国癌症杂志》
CAS
CSCD
北大核心
2022年第4期324-334,共11页
China Oncology
基金
国家自然科学基金(81673132,81972998)
江苏省卫健委项目(Z2018043)
江苏省青蓝工程(2019)
江苏省高职院校教师专业带头人高端研修项目资助(2021GRFX030)
校级自然科学基金(20204103)。
