摘要
目的探究不同浓度姜黄素对小鼠小胶质细胞(BV2细胞)低、中、高剂量锰暴露后存活率及TNF-α表达水平的影响。方法经预实验筛选出锰浓度和处理时间以及姜黄素干预浓度。设置空白对照组,低、中、高染锰组,低锰+姜黄素(低、中、高)组、中锰+姜黄素(低、中、高)组、高锰+姜黄素(低、中、高)组。采用MTT法测定吸光度值,并计算各组细胞存活率。用ELISA法测定姜黄素干预染锰BV2细胞后TNF-α的表达水平。结果与空白对照组相比,低、中、高单纯染锰组细胞活力显著降低(P<0.001);低剂量染锰组的BV2细胞在低浓度的姜黄素干预后细胞活力未见明显增加(P>0.05),但中、高剂量的姜黄素干预后均能使BV2细胞活力显著增加(P<0.05);中、高剂量染锰组的BV2细胞采用不同浓度的姜黄素进行干预后均能使细胞存活率显著增加,随姜黄素浓度的升高作用越明显,且各干预组之间也存在显著差异(P<0.05)。ELISA结果显示,染锰组TNF-α水平明显增高(P<0.001),姜黄素可显著降低染锰BV2细胞TNF-α的水平(P<0.001)。结论姜黄素能增加染锰BV2细胞的存活率且呈剂量依赖性,其机制可能与姜黄素抑制染锰BV2细胞炎症因子TNF-α的表达水平有关。
Objective o investigate the effects of different concentrations of curcumin on survival rate and TNF-αexpression level of mouse microglia(BV2 cells)after exposure to low,medium,and high doses of manganese.Methods Manganese concentration,treatment time and curcumin intervention concentration were screened by pre-experiment.Experimental groups:blank control group,pure low,medium,and high curcumin groups,low,medium,and high manganese groups,low manganese+curcumin(low,medium and high)group,medium manganese+curcumin(low,medium and high)group,high manganese+curcumin(low,medium and high)group.MTT method was used to determine the absorbance value and calculate the cell survival rate of each group.The expression level of TNF-αwas determined by ELISA after curcumin intervention in Mn BV2 cells.Results Compared with blank control group,the cell viability of BV2 was significantly decreased in low,medium,and high Mn groups(P<0.001).The cell viability of BV2 infected with low dose of manganese was not significantly increased by curcumin at low concentration(P>0.05)but was significantly increased by curcumin at medium and high dose(P<0.05).The survival rates of BV2 cells infected with medium and high dose of manganese were significantly increased with the increase of curcumin concentration,and there were significant differences among the intervention groups(P<0.05).ELISA results showed that the level of TNF-αin mn stained BV2 cells was significantly increased(P<0.001),and curcumin significantly decreased the level of TNF-αin Mn stained BV2 cells(P<0.001).Conclusion Curcumin can increase the survival rate of Mn BV2 cells in a dose-dependent manner,and the mechanism may be related to curcumin inhibiting the expression of TNF-αin Mn BV2 cells.
作者
韦黎平
赵才志
赵倩
杨力
戴婷
宋具鸿
赵华
Wei Liping;Zhao Caizhi;Zhao Qian(Toxicity Detection Center,Key Laboratory of Environmental Pollution and Disease Control,Ministry of Education,School of Public Health and Health, Guizhou Medical University,Guiyang,Guizhou 550025,China)
出处
《湘南学院学报（医学版）》
2022年第1期12-17,共6页
Journal of Xiangnan University（Medical Sciences）
基金
2020年国家级大学生创新创业训练计划项目(202010660030)。
