摘要
目的观察石菖蒲、远志及其配伍对氧化型低密度脂蛋白(ox-LDL)诱导人脐静脉内皮细胞(HUVECs)损伤情况的影响。方法2021年1月至8月,培养HUVECs,随机分为空白组、模型组和样品组,样品组中分别加入不同浓度(5、10、20 mg/ml)的石菖蒲、远志或石菖蒲+远志,对HUVECs增殖、相对活率试验、细胞凋亡、细胞周期、凋亡蛋白Bax和Bcl-2表达进行检测。采用单因素方差分析。结果石菖蒲、远志及其配伍均能减少HUVECs的凋亡[空白组(0.338±0.001),模型组(4.319±0.124),石菖蒲组(2.694±0.561),远志组(2.278±0.914),石菖蒲+远志组(2.250±0.699)];石菖蒲、远志及其配伍均能增加HUVECs周期(S+G2)%;远志、石菖蒲均能使凋亡相关蛋白Bax下调,同时抑制凋亡相关蛋白Bcl-2上调,远志+石菖蒲配伍下对Bax及Bcl-2蛋白的影响优于单味中药石菖蒲、远志。结论石菖蒲、远志及其配伍均能抑制ox-LDL诱导HUVECs损伤,其配伍作用强于单味中药。
Objective To investigate the effects of Acorus tatarinowii,Polygala tenuifolia,and their compatibility on ox-LDL-induced injury of human umbilical vein endothelial cells(HUVECs).Methods From January to August 2021,HUVECs were cultured,and were randomly divided into a blank group,a model group,and sample groups.The different concentrations(5,10,and 20 mg/ml)of Acorus tatarinowii,Polygala tenuifolia,and their compatibility were added into the sample groups.The HUVECs proliferation,relative viability test,apoptosis,cell cycle,and expressions of apoptotic proteins Bax and Bcl-2 were detected.One-way ANOVA was applied.Results Acorus tatarinowii,Polygala tenuifolia,and their ompatibility could reduce the apoptosis of HUVECs[the blank group(0.338±0.001),the model group(4.319±0.124),the Acorus tatarinowii group(2.694±0.561),the Polygala tenuifolia group(2.278±0.914),and the Acorus tatarinowii+Polygala tenuifolia group(2.250±0.699)],and increase HUVECs cycle(S+G2)%.Both Acorus tatarinowii and Polygala tenuifolia could down-regulate the apoptotic protein Bax and inhibit the up-regulation of the apoptotic protein Bcl-2,and their compatibility was better than Acorus tatarinowii and Polygala tenuifolia.Conclusion Acorus tatarinowii,Polygala tenuifolia,and their ompatibility can inhibit ox-LDL induced HUVECs injury,and their compatibility is stronger than single Chinese medicine.
作者
陈志宁
刘晓俊
唐梁
梁伟海
林国伟
Chen Zhining;Liu Xiaojun;Tang Liang;Liang Weihai;Lin Guowei(Department of Traditional Chinese Medicine,Guangzhou First People's Hospital,Guangzhou 511458,China)
出处
《国际医药卫生导报》
2022年第9期1199-1203,共5页
International Medicine and Health Guidance News
基金
广东省科技计划项目(2017A020213037)
广东省中医药局科研项目(20191266)。
关键词
石菖蒲
远志
配伍
人脐静脉内皮细胞
Acorus tatarinowii
Polygala tenuifolia
Compatibility
HUVECs
