摘要
为探讨生物质炭配施木灰对土壤碳固存和微生物群落的影响,通过118 d的室内培养试验,研究了单施生物质炭(B)、生物质炭配施木灰(B+W)处理下土壤碳固存、化学性质、酶活性、微生物群落组成的变化。结果表明:B、B+W处理均降低了CO_(2)-C释放速率和累积释放量,且B+W处理推迟CO_(2)释放速率峰值的出现;B、B+W处理均能提高土壤有机碳、无机碳含量。培养118 d时,B、B+W处理的微生物生物量碳高于对照,且B处理高于B+W处理;B+W处理提高了土壤pH与溶解性有机碳含量;B处理提高了土壤β-1,4-葡萄糖苷酶活性,B+W处理的β-1,4-木糖苷酶、β-1,4-葡萄糖苷酶活性分别较对照降低38.4%、15.3%,而蔗糖酶活性较对照提高61.4%。B处理对细菌丰富度和多样性无显著影响,却降低真菌Chao1、Ace指数;B+W处理虽降低了细菌丰富度和多样性,却能恢复真菌Chao1、Ace指数,并显著提高Shannon指数。培养118 d时,B处理提高了放线菌门相对丰度,降低了子囊菌门相对丰度;B+W处理的绿弯菌门及担子菌门相对丰度提高,拟杆菌门相对丰度降低。冗余分析发现,B、B+W处理通过改变土壤化学性质影响土壤微生物群落结构,土壤有机碳与pH分别是影响细菌、真菌群落结构最显著的土壤因子,且B+W处理在降低石灰性土壤碳矿化、维持真菌群落稳定性方面效果更佳。
In order to explore the effects of biochar combined with wood ash on soil carbon sequestration and microbial community,the changes of soil carbon sequestration,soil chemical properties,enzyme activities and microbial community compositions under biochar(B)and biochar combined with wood ash(B+W)treatments were studied through 118-day laboratory incubation.The results showed that both B and B+W treatments decreased the respiration rate and cumulative CO_(2)-C respiration,and B+W treatment delayed the peak of CO_(2)-C respiration.B and B+W treatments could increase the content of soil organic carbon and inorganic carbon.On day 118,the microbial biomass carbon of B and B+W treatments was higher than that of control,and that of B treatment was higher than that of B+W treatment,B+W treatment significantly increased the content of soil dissolved organic carbon and pH.B treatment increased the activities ofβ-1,4-glucosidase,the activities ofβ-1,4-xylosidase,β-1,4-glucosidase in B+W treatment were decreased by 38.4%,15.3%,and the activity of invertase was increased by 61.4%respectively,as compared to control.B treatment had no significant effect on bacterial richness and diversity,but decreased Chao1 and Ace index of fungi;B+W treatment decreased the bacterial richness and diversity,but restored the fungal Chao1 and Ace index,and significantly increased Shannon index.On day 118,B treatment increased the relative abundance of Actinomycetes and decreased the relative abundance of Ascomycetes,B+W treatment increased the relative abundance of Chloroflexi and Basidiomycota,but decreased the relative abundance of Bacteroidetes.Redundancy analysis showed that biochar and biochar combined with wood ash affected soil microbial community by changing the soil chemical properties,soil organic carbon and pH were the most significant soil factors affecting bacterial and fungal community structure,respectively,B+W treatment showed better performance in reducing carbon mineralization in calcareous soils and maintaining the stability of the fungal community.
作者
王丽
赵惠丽
赵英
WANG Li;ZHAO Huili;ZHAO Ying(College of Resources and Environmental Engineering,Ludong University,Yantai,Shandong 264025,China)
出处
《土壤》
CAS
CSCD
北大核心
2022年第2期320-328,共9页
Soils
基金
山东省自然科学基金项目(ZR2020QD115)
山东省杰出青年基金项目(ZR2019JQ12)资助。
关键词
碳固存
微生物群落
生物质炭
木灰
石灰性土壤
Carbon sequestration
Microbial community
Biochar
Wood ash
Calcareous soil
