摘要
【目的】研究异源胞质甘油醛-3-磷酸脱氢酶2(cytoplasm glyceraldehyde-3-phosphate dehydrogenase,GAPC2)对番茄果实成熟的影响。【方法】构建“红颜”草莓FaGAPC2过表达载体,采用瞬时过表达技术在樱桃番茄“罗纳”的破色期过表达FaGAPC2。【结果】FaGAPC2的表达量为对照组的2.39倍,过表达FaGAPC2能够显著抑制番茄果皮颜色变红并显著提高其硬度(3.76N)、降低可溶性糖含量,其类胡萝卜素含量和总叶绿素含量分别是对照组的0.37倍和1.5倍。进一步通过实时荧光定量分析结果表明,过表达FaGAPC2较对照能显著降低乙烯合成途径中ACO1(12.89倍)、ACO3(3.87倍)和ERF1(3.51倍);类胡萝卜合成途径中PSY1(12.39倍)、CYC-B(1.49倍);果实成熟相关基因E4(4.48倍)、PG(41.35倍)和MADS转录因子RIN(6.67倍)的表达量,其中果实成熟相关基因E8、LOXB相比于对照表达量几乎为零。【结论】表明FaGAPC2作为一个负调节因子参与番茄果实成熟的调控。
【Objective】This paper aims to study the effect of heterologous cytoplasmic glyceraldehyde-3-phosphate dehydrogenase 2(GAPC2)gene on tomato fruit ripening.【Method】The strawberry(Fragaria×ananassa‘Benihoppe’)FaGAPC2 overexpression vector was constructed,and transient overexpression technology was used to overexpress FaGAPC2 during the breaking period of cherry tomato(Solanum lyco⁃persicum‘luona’).【Result】The experimental results showed that the expression level of FaGAPC2 was 2.39-fold that of the control.Overexpression of FaGAPC2 can significantly inhibit the coloring of tomato peel and the accumulation of the total soluble solids content,while increase its hardness to 3.76N.Mean⁃while,the contents of carotenoid and total chlorophyll were about 0.37-fold and 1.5-fold higher than that of the control,respectively.Further real-time fluorescence quantitative analysis results showed that over⁃expression of FaGAPC2 can significantly reduce the expression of 1-aminocyclopropane-1-carboxylate oxidase1(ACO1,12.89-fold),and 1-aminocyclopropane-1-carboxylate oxidase3(ACO3,3.87-fold),and ethylene-responsive element factors1(ERF1,3.51-fold)in the ethylene synthesis pathway;phytone synthase1(PSY1,12.39-fold),and chromoplast-specific lycopene-β-cyclase(CYC-B,1.49-fold)in the carotenoid synthesis pathway;fruit ripening-associated genes:peptide methionine sulfoxide reduc⁃tase4(E4,4.48-fold),polygalacturonase(PG,41.35-fold)and the MADS transcription factor ripening inhibitor(RIN,6.67-fold).In note,the expression level of fruit ripening-related genes 1-aminocyclopro⁃pane-1-carboxylate oxidase-like(E8)and lipoxygenase(LOXB)were almost zero compared to the con⁃trol.【Conclusion】It showed that FaGAPC2 is involved in the regulation of tomato fruit ripening as a negative regulator.
作者
杨敏
龙宇
王良新
刘晓阳
侯国艳
蒋语嫣
罗娅
YANG Min;LONG Yu;WANG Liangxin;LIU Xiaoyang;HOU Guoyan;JIANG Yuyan;LUO Ya(College of Horticulture,Sichuan Agricultural University,Chengdu 611130,China)
出处
《四川农业大学学报》
CSCD
北大核心
2022年第2期156-162,171,共8页
Journal of Sichuan Agricultural University
基金
国家自然科学基金项目(3180817)
四川省科技厅项目(2018NZ0126)。
