期刊文献+

超高效液相色谱-串联质谱法同时测定黑巧克力中4种牛奶过敏原蛋白 被引量:3

Determination of four milk allergen proteins in dark chocolates by ultra-performance liquid chromatography-tandem mass spectrometry
原文传递
导出
摘要 目的建立黑巧克力中4种牛奶过敏原蛋白的超高效液相色谱-串联质谱检测方法。方法从Uniprot数据库筛选牛奶主要过敏原蛋白的特异肽段,用Skyline工具模拟胰酶酶切,确定各肽段的多反应监测(multiple reaction monitoring,MRM)方法。50 mmol/L碳酸氢铵(含2 mol/L尿素)提取巧克力中目标蛋白,14000 r/min、4℃离心除脂,胰酶酶解上清液,固相萃取柱净化,Peptide BEH C_(18)(130?,2.1 mm×100 mm,1.7μm)色谱柱分离,以乙腈和水[均含0.1%(V/V)甲酸]为流动相进行梯度分离,标准加入法定量。结果4条特异肽段FFVAPFPEVFGK、NAVPITPTLNR、AVPYPQR和ALPMHIR可分别作为αS1-酪蛋白、αS2-酪蛋白、β-酪蛋白、β-乳球蛋白的定量肽段。其质量浓度与质谱响应峰面积呈现良好线性关系(R^(2)>0.99),方法的检出限和定量限分别为50~100和160~350μg/kg,加标回收率为60.6%~116.7%,相对标准偏差均小于20%(n=6)。结论该方法灵敏、准确、高效,适于检测黑巧克力中4种牛奶过敏原蛋白。 OBJECTIVE To develop a new method for the simultaneous determination of four milk allergen proteins in dark chocolates by ultra-performance liquid chromatography-tandem mass spectrometry.METHODS The specific peptides of main allergen proteins in milk were screened in Uniprot database.Skyline tool was used to simulate the trypsin digestion.Multiple reaction monitoring(MRM)in positive electrospray ionization mode was performed.Milk allergen proteins in chocolates were extracted by 50 mmol/L ammonium bicarbonate(containing 2 mol/L urea)and centrifuged at 14000 r/min with a temperature of 4℃to remove fat.The supernatant was enzymatic hydrolyzed by trypsin for 12 h.The enzymatic hydrolysate was purified by solid-phase extraction.The purified solution was separated on a Peptide BEH C;column(130?,2.1 mm×100 mm,1.7μm)using a gradient mobile phase of acetonitrile and water(both containing 0.1%formic acid(V/V)).Quantitative analysis was made by standard addition method.RESULTS Four specific peptide FFVAPFPEVFGK,NAVPITPTLNR,AVPYPQR and ALPMHIR could be used as quantitative peptides ofαS1-casein,αS2-casein,β-casein andβ-lactoglobulin in chocolates.Good linear relationships between the peak areas of mass spectrometry and the mass concentration could be obtained with correlation coefficients(R;)above 0.99.The limits of detection and quantitation of the above four peptides were in the ranges of 50-100μg/kg and 160-350μg/kg,respectively.The recoveries were ranging from 60.6%to 116.7%with relative standard deviations(n=6)all lower than 20%.CONCLUSION This method is sensitive,accurate and efficient,which is suitable for the analysis of four types of milk allergen proteins in dark chocolate.
作者 刘晋源 杨蕴嘉 姚凯 杨奕 邵兵 丁晓静 Liu Jinyuan;Yang Yunjia;Yao Kai;Yang Yi;Shao Bing;Ding Xiaojing(School of Publish Health,Capital Medical University,Beijing 100069,China;Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning,Beijing Center for Disease Prevention and Control,Beijing Center for Preventive Medicine Research,Beijing 100013,China)
出处 《卫生研究》 CAS CSCD 北大核心 2022年第2期286-292,共7页 Journal of Hygiene Research
基金 北京市科技计划项目(No.Z181100009318009)。
关键词 巧克力 过敏原蛋白 超高效液相色谱-串联质谱 牛奶蛋白 chocolate allergen protein ultra-performance liquid chromatography-tandem mass spectrometry milk protein
  • 相关文献

参考文献8

二级参考文献158

  • 1朱倩倩,吴序栎,肖杰,成小娟,lujun,刘志刚,徐宏.腰果过敏原Ana o 2结构及抗原表位预测[J].食品与生物技术学报,2012,31(2):141-145. 被引量:5
  • 2张天裘.儿童食物变态反应主要影响因素的研究进展[J].国外医学(儿科学分册),1995,22(2):90-92. 被引量:6
  • 3焦凌梅,袁唯.改善山羊乳风味的方法研究[J].中国乳业,2006(6):56-58. 被引量:39
  • 4于冬梅,翟凤英,张兵,赵丽云,王惠君,刘爱东,于文涛,贾凤梅,王志宏,杨清俊,焦欣然,赵冬丽,易国勤.北京市和湖北省儿童青少年零食消费现况调查分析[J].中国健康教育,2007,23(6):403-406. 被引量:27
  • 5CATHRO J, HILLIAM M. Children's eating habits in european: indepth consumer and market analysis[M]. Leatherhead: Leatherhead Food Res Assoc, 1994.
  • 6WOLFE W S, CAMPBELL C C. Food pattern, diet quality, and related characteristics of school children in New York State [ J ]. J Am Dietri Assoc, 1993,93 : 1280-1284.
  • 7JAHNS L, SIEGA-RIZ A M, Popkin B M. The increasing prevalence of snacking among US children from 1977 to 1996[J]. Pediatric, 2001,138 (4) :493-498.
  • 8PAULA Ziegler, RONETTE Briefel, Michael Ponza, et al. Nutrient intakes and food patterns of toddlers' lunches and snacks: influence of locatian[J]. J Am Dietr Assoc, 2006, 1(suppl) :124-134.
  • 9RAYNAL-LJUTOVAC K, PARK Y W, GAUCHERON F, et al. Heat Stability and Enzymatic Modifications of Goat and Sheep milk[J]. Small Ruminant Research, 2007, 68:207 - 220.
  • 10MIKALLES B, BARTOLOME B, RAMOS M, AMIGO L. Detemi- nation of Whey Protein to Total Protein Ratio in UHT Milk Using Fourth Derivative Spectroscopy[J]. International Dairy Journal,2000, 10: 191-197.

共引文献96

同被引文献31

引证文献3

二级引证文献3

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部