摘要
采用微波辅助水蒸气蒸馏法提取金盏花精油,以得率为评价指标,在单因素实验的基础上通过正交试验对精油提取工艺进行优化,随后通过气相色谱-质谱(GC-MS)对金盏花精油化学成分进行分析,最后以DPPH自由基、羟自由基、ABTS+自由基清除能力为指标评价金盏花精油的抗氧化效果。结果表明,微波辅助水蒸气蒸馏法提取金盏花精油的最佳工艺条件为:NaCl浓度3%、料液比1:30 g/mL、微波功率240 W,在此条件下精油得率为0.156%。通过GC-MS分析,金盏花精油共被鉴定出25种组分,占总成分的97.94%,主要为醇类和萜烯类化合物。在抗氧化试验中,金盏花精油对DPPH自由基的清除能力较弱,对羟自由基和ABTS+自由基的清除能力较强,总体上具有明显的抗氧化活性并有良好的量效关系。本研究将为金盏花精油的大规模生产提供较为可靠的工艺参数,并为评价其抗氧化活性提供基础数据和参考。
The essential oil from Calendula officinalis L. was extracted by microwave-assisted steam distillation. Taking the yield as the evaluation index, the extraction process of essential oil was optimized by orthogonal experiment on the basis of single factor test. The chemical composition of the essential oil was analyzed by gas chromatography-mass spectrometry(GC-MS). The antioxidant effect of the essential oil was evaluated by the scavenging activity of DPPH free radical,hydroxyl radical and ABTS+ free radical. The results showed that the optimum conditions for extracting the essential oil by microwave-assisted steam distillation were as follows: NaCl content 3%, ratio of material to liquid 1:30 g/mL, microwave power 240 W. Under these conditions, the yield of the essential oil was 0.156%. By GC-MS analysis, a total of 25components were identified in the essential oil, accounting for 97.94% of the total components, mainly alcohols and terpenes. In the antioxidant test, the scavenging ability of the essential oil to DPPH free radical was weak. It had strong scavenging ability to hydroxyl radical and ABTS+ radical. In general, it had obvious antioxidant activity and a good doseeffect relationship. This study would provide more reliable technological parameters for large-scale production of Calendula officinalis L. essential oil, and provide basic data and reference for the evaluation of its antioxidant activity.
作者
刘欢
赵巨堂
何力
罗海涛
熊文俊
邹恩凯
LIU Huan;ZHAO Jutang;HE Li;LUO Haitao;XIONG Wenjun;ZOU Enkai(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China;Tobacco Jiangxi Industrial Co.,Ltd.,Nanchang 330096,China)
出处
《食品工业科技》
CAS
北大核心
2022年第10期180-188,共9页
Science and Technology of Food Industry
基金
国家自然科学基金青年项目(31901648)
国家自然科学基金面上项目(31770861)
国家自然科学基金地区科学基金项目(31960464)。
关键词
金盏花精油
微波提取
气相色谱-质谱法(GC-MS)
化学成分
抗氧化活性
Calendula officinalis L.essential oil
microwave extraction
gas chromatography-mass spectrometry(GC-MS)
chemical components
antioxidant activity
