摘要
目的观察电针联合丹参多酚酸盐对急性心肌梗死大鼠心功能及药物组织分布的影响。方法采用冠状动脉左前降支结扎术制备急性心肌梗死(AMI)大鼠模型。将造模成功大鼠随机分为模型组、药物组、电针组和电针联合药物组(针药组),每组9只。同时设9只大鼠为空白组。药物组给予尾静脉注射丹参多酚酸盐(30 mg/kg),电针组予双侧内关、心俞穴电针干预,针药组采用电针联合丹参多酚酸盐干预,每天1次,连续7天。空白组、模型组仅束缚,不进行治疗干预。采用心脏超声评价大鼠左室射血分数(LVEF)和左室短轴缩短率(LVFS);TTC染色观察心肌梗死面积;检测血清肌酸激酶同工酶(CK-MB)、肌钙蛋白(cTnT)及乳酸脱氢酶(LDH)水平;高效液相—质谱联用法检测药物组和针药组大鼠血浆及各脏器组织中丹参多酚酸盐主要成分——丹酚酸B的含量。结果与空白组比较,模型组大鼠LVEF、LVFS均降低(P<0.01),梗死面积增大(P<0.05),血清CK-MB、cTnT、LDH均升高(P<0.01)。干预后与模型组比较,针药组大鼠LVEF、LVFS升高(P<0.01),药物组、针药组大鼠心肌梗死面积减小(P<0.01),药物组、电针组和针药组大鼠血清CK-MB、cTnT、LDH均降低(P<0.01),其中针药组血清cTnT低于药物组(P<0.05),CK-MB、cTnT和LDH较电针组降低更明显(P<0.01);与药物组比较,针药组心脏组织丹酚酸B含量增高(P<0.05)。结论电针内关、心俞穴联合丹参多酚酸盐能够改善AMI大鼠心功能,其作用与电针特异性增加心脏组织丹酚酸B含量有关。
Objective To observe the effects of electroacupuncture(EA)combined with salvianolate(SAL)on cardiac function and drug tissue distribution in rats with acute myocardial infarction(AMI).Methods The AMI rat model was established by ligation the left anterior descending branch of coronary artery.The successful model of rats were randomly divided into model group,SAL group,EA group,and electroacupuncture combined with salvianolate group(EA-SAL group),with 9 rats in each group.Besides,another 9 rats were recruited as a blank group.The SAL group was given intravenous injection of salvianolate(30 mg/kg),the EA group was given bilateral electroacupuncture intervention at Neiguan(PC6)and Xinshu(BL15)acupoints,and the EA-SAL group was given electroacupuncture combined with salvianolate for intervention.Treatment was administered once a day for 7 days.The blank group and model group were only restrained without any therapeutic intervention.Cardiac ultrasound was performed for the evaluation of left ventricular ejection fraction(LVEF)and left ventricular fractional shortening(LVFS)in rats,TTC staining was implemented to observe the myocardial infarction area.In the meantime,the levels of serum creatine kinase-MB(CK-MB),cardiac troponin T(cTnT)and lactate dehydrogenase(LDH)were detected.Moreover,high performance liquid phase-mass spectrometry(HPLC)was utilized for the detection of the content of salvianolic acid B(Sal B),the principal component of salvianolate in plasmaand organ tissues of rats in the drug group and the combination group.Results Compared with the blank group,the LVEF and LVFS decreased(P<0.01),infarct size increased(P<0.05),serum levels of CK-MB,cTnT,and LDH also increased(P<0.01)in the model group.After the intervention,compared with the model group,the LVEF and LVFS increased in the EA-SAL group(P<0.01).The myocardial infarction area decreased in the SAL group and the EA-SAL group(P<0.01).And the serum CKMB,cTnT and LDH decreased in the SAL group,EA group and EA-SAL group(P<0.01),respectively,among them,the serum cTnT of the EA group was lower than that of the SAL group(P<0.05).In addition,levels of serum CK-MB,cTnT and LDH were reduced more significantly(P<0.01)than those in EA group.Compared with the SAL group,the content of Sal B of the heart tissue was increased in the EA-SAL group(P<0.05).Conclusion Electroacupuncture at Neiguan(PC6)and Xinshu(BL15)acupoints combined with salvianolate could markedly improve the heart function of AMI rats,and the effect was correlated to the specific increase of Sal B content in heart tissue by electroacupuncture treatment.
作者
安琪
赵佳
刘佩东
刘阳阳
郭永明
郭义
AN Qi;ZHAO Jia;LIU Pei-dong;LIU Yang-yang;GUO Yong-ming;GUO Yi(Research Center of Experimental Acupuncture Science,Tianjin University of Traditional Chinese Medicine,Tianjin 300163;The Second Clinical Medical College,Guangzhou University of Chinese Medicine,Guangzhou 500120;Department of Acupuncture and Moxibustion,Jungar Banner Mongolia Hospital,Inner Mongolia 010300;Department of Acupuncture and Moxibustion,Shenzhen Baoan Hospital of Traditional Chinese Medicine,Guangdong 518100;National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion,Tianjin 300381;School of Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617)
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2022年第3期334-340,共7页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家重点研发计划(No.2019YFC1709003)
国家自然科学基金面上项目(No.81873369)。
关键词
针药联用
电针
丹参多酚酸盐
心肌梗死
药物分布
acupuncture combined with drug
electroacupuncture
Salvianolate
myocardial infarction
drug distribution
