摘要
目的目前脓毒症动物模型主要通过盲肠结扎穿孔法引起腹腔混合细菌感染建立,而内科临床上更为常见的是呼吸道细菌感染引发的脓毒症,因此需要建立肺部感染相关脓毒症动物模型。方法大鼠按照金黄色葡萄球菌、铜绿假单胞菌菌种及细菌悬液浓度的不同分为S-Cont组、S-0.75组、S-1.5组、S-3组、S-6组和P-Cont组、P-1组、P-2组、P-4组、P-8组共10组;对照组以生理盐水0.2 ml滴鼻,各实验组分别以0.75×10^(8) CFU/ml、1.5×10^(8) CFU/ml、3×10^(8) CFU/ml、6×10^(8) CFU/ml的金黄色葡萄球菌悬液或1×10^(8) CFU/ml、2×10^(8) CFU/ml、4×10^(8) CFU/ml、8×10^(8) CFU/ml的铜绿假单胞菌悬液滴鼻法感染大鼠。检测各组大鼠感染前后的体温(T)、血压(BP)、心率(HR)和感染后血乳酸(Lac)、降钙素原(PCT)水平;并通过苏木精-伊红染色法(HE)观察各组大鼠肺部感染情况。结果S-1.5组、S-3组、S-6组及P-8组BP水平降低(均P<0.05);金黄色葡萄球菌各实验组Lac及PCT均较S-Cont组升高(均P<0.01),除S-3组与S-6组差异无统计学意义(P>0.05)外,随浓度增加呈递增趋势(P<0.05);铜绿假单胞菌各实验组Lac及PCT均较P-Cont组升高(均P<0.01),除P-4组与P-8组Lac比较差异无统计学意义(P>0.05)外,随浓度增加呈递增趋势(均P<0.05);HE染色提示各实验组大鼠肺部均可见到不同程度的炎症浸润表现。结论分别以金黄色葡萄球菌悬液3×10^(8) CFU/ml、铜绿假单胞菌悬液4×10^(8) CFU/ml滴鼻法感染大鼠,可以建立较为稳定的单种细菌肺部感染诱发的脓毒症大鼠模型;且金黄色葡萄球菌悬液3×10^(8) CFU/ml滴鼻可以形成较为稳定的脓毒性休克大鼠模型。
Objective Animal models of sepsis are mainly established by cecal ligation and puncture which causes mixed bacterial infections in the abdominal cavity.However in internal clinic,sepsis is more common to be caused by respiratory bacterial infections.Therefore,it is necessary to establish animal models of sepsis caused by lung Infection.Methods According to the concentration of Staphylococcus aureus(S.aureus)suspension and Pseudomonas aeruginosa(P.aeruginosa)suspension,Sprague Dawley(SD)rats were equally divided into 10 groups,including S-Cont group,S-0.75 group,S-1.5 group,S-3 group,S-6 group and P-Cont group,P-1 group,P-2 group,P-4 group,P-8 group.Rats in the control group were treated with normal saline nasal drip.Rats in each experimental group were infected by nasal dripping bacterial suspension with 0.75×10^(8) CFU/ml,1.5×10^(8) CFU/ml,3×10^(8) CFU/ml,6×10^(8) CFU/ml of S.aureus suspension or 1×10^(8) CFU/ml,2×10^(8) CFU/ml,4×10^(8) CFU/ml,8×10^(8)CFU/ml P.aeruginosa suspension.Our study detected the body temperature(T),blood pressure(BP),heart rate(HR)of rats in each group before and after infection,as well as blood lactic acid(Lac)and procalcitonin(PCT)level after infection.The lung infections of rats in each group were observed by hematoxylin-eosin(HE)staining.Results The blood pressure(BP)of S-1.5 group,S-3 group,S-6 group and P-8 group was lower than before infection(all P<0.05).The Lac and PCT of each S.aureus experimental group were higher than that of the S-Cont group(all P<0.01);and they showed an increasing trend with the increase of the bacterial suspension concentration(P<0.05),except for the S-3 and S-6 group(P>0.05).The Lac and PCT of each P.aeruginosa experimental group were higher than that of the P-Cont group(all P<0.01);and they showed an increasing trend with the increase of the bacterial suspension concentration(all P<0.05),except for the Lac in the P-4 group and P-8 group(P>0.05).HE staining showed that different degrees of inflammatory infiltration can be seen in the lungs of the experimental rats in each group.Conclusions Infection of rats by nasal dripping with 3×10^(8) CFU/ml of S.aureus suspension or 4×10^(8) CFU/ml of P.aeruginosa suspension could establish relatively stable rat sepsis model induced by lung bacterial infection,of which the former could also establish a relatively stable septic shock model.
作者
吴彩军
晏军
刘锦
刘艺
南富耀
苏家卉
马林沁
Wu Caijun;Yan Jun;Liu Jin;Liu Yi;Nan Fuyao;Su Jiahui;Ma Linqin(Department of Emergency Medicine,Dongzhimen Hospital of Beijing University of Chinese Medicine,Beijing 100700,China;Institute of Sepsis,Beijing University of Chinese Medicine,Beijing 100700,China;Dongzhimen Hospital of Beijing University of Chinese Medicine,Beijing 100700,China)
出处
《中国医师杂志》
CAS
2022年第4期490-495,共6页
Journal of Chinese Physician
基金
中央高校基本科研业务费专项资金资助(2018-JYBZZ-XJSJJ024)
北京中医药大学东直门医院特殊人才引进科研启动费专项(2018RC01)。
