摘要
[目的]本文旨在通过测定关键生育期不同抗旱胁迫程度下不同品种玉米抗氧化酶活性、脯氨酸(Pro)及丙二醛(MDA)含量等指标,阐明玉米在不同干旱胁迫程度下的生理应激响应,为玉米抗旱稳产提供理论基础。[方法]以‘金庆707’‘嫩单19’及‘富单16’3个不同耐旱能力的玉米品种为试验材料,通过设置抗旱棚微区试验,于玉米拔节期、抽雄期及灌浆期分别设置正常供水(CK)、轻旱(LS)、中旱(MS)、重旱(SS)及持续干旱(S)处理5个水分梯度对玉米生理指标进行测定。[结果]随干旱胁迫程度的不断增强,玉米叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性均呈先升高后降低的趋势,并在中度干旱[田间持水量的(45±5)%]时达到峰值。抽雄期玉米生长速率较快,干旱胁迫条件下3种抗氧化酶活性也最高,3个品种玉米酶活性从高到低分别为‘金庆707’‘嫩单19’‘富单16’。随干旱胁迫的增强,不同品种玉米叶片Pro含量显著升高,其中抽雄期Pro含量最高,最高可达到2101.12 ng·g^(-1)。玉米叶片MDA含量在灌浆期最高,胁迫程度严重时MDA含量显著高于对照组,其中玉米品种‘金庆707’MDA含量最低,玉米品种耐旱性越强,MDA含量越低。[结论]抽雄期干旱胁迫影响产量,土壤水分维持在田间持水量的50%以上时,玉米通过增强抗氧化酶活性及脯氨酸含量来保护膜系统,在水分低于田间持水量的50%时则较易受到胁迫影响。
[Objectives]The purpose of this study was to clarify the physiological response of maize under different drought stress levels by measuring the antioxidant enzyme activities,proline(Pro)and malondialdehyde(MDA)contents of different maize varieties under different drought stress levels at key growth stages,and to provide a theoretical basis for drought resistance and stable yield of maize.[Methods]Three maize varieties with different drought tolerance,‘Jinqing 707’‘Nendan 19’and‘Fudan 16’,were used as experimental materials.Five water gradients of normal water supply(CK),light drought(LS),medium drought(MS),severe drought(SS)and continuous drought(S)treatments were set at jointing stage,tasseling stage and filling stage of maize.[Results]With the increasing of drought stress,superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activities in maize leaves showed a trend of first increasing and then decreasing.At the same time,it reached the peak in moderate drought[(45±5)%of field water capacity].The growth rate of maize was faster at heading stage,and the activities of three antioxidant enzymes were also the highest under drought stress.Among them,three varieties from high to low were‘Jinqing 707’‘Nendan 19’and‘Fudan 16’,respectively.With the increment of drought stress,the Pro content in maize leaves of different varieties increased significantly,and the highest Pro content was 2101.12 ng·g^(-1) at tasseling stage.Compared with the control,the content of Pro increased most significantly under continuous drought.The content of MDA was the highest in the filling stage.When the stress was serious,the content of MDA was significantly higher than that of the control group,but the stronger the drought tolerance of maize varieties,the lower the content.[Conclusions]At tasseling stage drought stress affected yield more easily.When the soil moisture maintained more than 50%of field capacity,corn plant can enhance the antioxidant enzyme activity and Pro content.When the soil moisture maintained was less than 50%of field capacity,the corn plant were easily affected by stress.
作者
崔蓉
柳鑫婧
李锦秀
王呈玉
张欣宇
常洪艳
刘淑霞
CUI Rong;LIU Xinjing;LI Jinxiu;WANG Chengyu;ZHANG Xinyu;CHANG Hongyan;LIU Shuxia(College of Resource and Environmental Science/Key Laboratory of Soil Resource Sustainable Utilization forJilin Province Commodity Grain Bases,Jilin Agricultural University,Changchun 130118,China)
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2022年第3期545-552,共8页
Journal of Nanjing Agricultural University
基金
吉林省重点研发计划项目(20200403069SF)。
关键词
玉米
耐旱性
干旱胁迫程度
抗氧化特性
maize
drought tolerance
degree of drought stress
oxidation resistance
