摘要
目的评价在生化分析仪上应用胶乳免疫比浊法检测总前列腺特异性抗原(total prostate specific antigen,TPSA)的临床应用价值。方法对试剂盒的各项性能指标进行验证,留取标本对胶乳免疫比浊法结果与化学发光法测定结果进行比较,并对两种方法结果进行线性回归分析。结果精密度、正确度、线性范围、可报告范围、参考范围验证均符合要求,胶乳免疫比浊法样本结果与化学发光法相比较,相关性R^(2)=0.9769。结论胶乳免疫比浊法检测TPSA与发光法检测TPSA结果相符,可极大降低检测成本,扩大适用人群。
Objective To evaluate the clinical value of latex immunoturbidimetry in the detection of total prostate specific antigen(tPSA)using the biochemical analyzer.Methods We evaluated the performance indicators of the kit and prepared samples to compare the results detected by latex immunoturbidimetric method and chemiluminescence method.Results The precision accuracy,linear range and reportable range all met the requirements.The results of latex immunoturbidimetry were compared with that of chemiluminescence,and the correlation R2 was 0.9769.Conclusion The results of TPSA detected by latex immunoturbidimetry are consistent with those detected by luminescence method.Our study suggests that this method can greatly reduce the testing cost and expand the applicable population.
作者
黄素玲
张美娜
孙淑贤
杨晨敏
王京楠
魏晨玲
HUANG Suling;ZHANG Meina;SUN Shuxian;YANG Chenmin;WANG Jingnan;WEI Chenling(Shijiazhuang Hospital of Traditional Chinese Medicine,Shijiazhuang 050000,China)
出处
《标记免疫分析与临床》
CAS
2022年第3期501-505,共5页
Labeled Immunoassays and Clinical Medicine
基金
科技部国家重点研发计划(编号:2019YFF0216502)。
