摘要
目的探讨非综合征型耳聋患者血浆中miRNA-96和miRNA-183的表达水平,揭示两种miRNA的改变是否与耳聋存在关系。方法选取2019年3月-2021年10月于浙江省温州市人民医院就诊的非综合征型耳聋患者30例为耳聋组,听力正常30例为正常组。利用实时荧光RT-PCR技术检测两组血浆中miRNA-96和miRNA-183的表达水平,并对30例耳聋患者基因突变位点进行统计分析。结果耳聋组miRNA-96与正常组相比表达水平差异有统计学意义(P<0.05),为表达下调;miRNA-183与正常组相比表达水平差异有统计学意义(P<0.01),也为表达下调。耳聋基因突变位点统计显示,本研究中30例最常见的突变位点为GJB2235delC,其次为SLC26A4 IVS 2-7 A>G。结论本研究显示非综合征型耳聋患者与正常人血浆含有的miRNA-96和miRNA-183表达存在差异,提示两种miRNA与听力损失有一定的相关性。
Objective Analyze the expression levels of miRNA-96 and miRNA-183 in plasma of patients with non syndromic deafness,and reveal whether the changes of the two miRNAs are related to deafness.Methods 30 patients with non syndromic deafness treated in Wenzhou People's Hospital from March 2019 to October 2021 were selected as the experimental group and 30 normal hearing people as the control group.The expression levels of miRNA-96 and miRNA-183 in plasma of the two groups were detected by real-time fluorescent RT-PCR,and the expression levels of miRNA in plasma of the two groups were compared;The gene mutation sites of 30 deaf patients were statistically analyzed.Results The expression level of miRNA-96 in non syndromic deafness patients was significantly lower than that in normal subjects(P<0.05);The expression level of miRNA-183 was significantly lower than that of normal subjects(P<0.01).The statistics of deafness gene mutation sites show that the most common mutation site in 30 patients in this study is GJB2235delc,followed by SLC26A4 IVS 2-7 A>G.Conclusion This study shows that there are differences in the expression of miRNA-96 and miRNA-183 in plasma between patients with non syndromic deafness and normal people,suggesting that there is a certain correlation between the two miRNAs and hearing loss.
作者
夏雯丽
戴显宁
许锴
王晗
童郁
XIA Wenli;DAI Xianning;XU Kai;WANG Han;TONG Yu(Wenzhou People's Hospital,Wenzhou 325000,China)
出处
《浙江实用医学》
2021年第4期300-303,333,共5页
Zhejiang Practical Medicine
基金
温州市基础性科研项目(Y20190468)。
