摘要
目的观察三乙醇胺(TEOA)对弥漫大B细胞淋巴瘤(DLBCL)细胞生物特性的影响,旨在为DLBCL的临床治疗策略提供依据。方法DLBCL细胞株OCI-LY3源于中国科学细胞库,随机数字表法简单随机分为对照组和观察组,每组分别设置5个复孔,观察组加入不同浓度(5、10、15、20、25、30、35、40、45、50μmol/L)的TEOA于细胞培养箱培养12 h,采用Graph Pad Prism 5软件计算2组DLBCL细胞存活率,流式细胞术检测DLBCL细胞的凋亡和侵袭情况,MTT法检测DLBCL细胞的增殖情况,Western blotting检测蛋白相对表达量,比较2组细胞活性氧(ROS)水平,收集2组研究数据并录入SPSS 20.0统计学软件进行分析。结果TEOA作用后24、48、72 h观察组细胞增殖率为(39.24±3.95)%、(57.08±5.87)%、(114.08±14.69)%,明显低于对照组的(49.49±5.02)%、(95.12±9.64)%、(210.45±25.62)%,差异有统计学意义(均P<0.05);观察组穿膜细胞数[(38.14±3.77)个]明显低于对照组[(92.25±9.24)个],而细胞凋亡率[(12.14±1.24)%]明显高于对照组[(3.64±0.36)%],差异有统计学意义(P<0.05);TEOA可增加DLBCL OCI-LY3细胞内ROS的水平,并有效上调OCI-LY3中p38、p-Chk1、p-Chk2、γ-H2AX的表达水平。结论TEOA可抑制细胞增殖和侵袭,同时有效诱导细胞凋亡及ROS的表达,TEOA作用于DLBCL OCI-LY3细胞可促进p38、p-Chk1、p-Chk2、γ-H2AX的表达,并诱导p38 MAPK通路的激活。
Objective To observe the effect of triethanolamine(TEOA)on the biological characteristics of diffuse large B-cell lymphoma(DLBCL)cells,so as to provide a basis for clinical treatment of the disease.Methods DLBCL cell lines OCI-LY3 were provided by the cell bank of the Chinese Academy of Sciences.They were randomised into the control group and observation group by random number table method,with five parallel holes in each group.Different concentrations(5,10,15,20,25,30,35,40,45,50μmol/L)of TEOA were added into the observation group and then cultured for 12 h.The GraphPad Prism 5 software was used to calculate the survival rates of DLBCL cells in both groups,flow cytometry was used to detect the apoptosis and invasion of DLBCL cells,the MTT method was used to detect the proliferation of DLBCL cells,and Western Blotting was used to detect the relative protein expression.The levels of reactive oxygen species(ROS)in both groups were compared,and research data were collected and input into the SPSS 20.0 statistical software for statistical analysis.Results After 24,48 and 72 h of TEOA treatment,the cell proliferation rates in the observation group[(39.24±3.95)%,(57.08±5.87)% and(114.08±14.69)%]were significantly lower than those in the control group[(49.49±5.02)%,(95.12±9.64)% and(210.45±25.62)%,all P<0.05].The observation group had significantly smaller number of transmembrane cells(38.14±3.77)and significantly higher apoptosis rate[(12.14±1.24)%]than the control group[92.25±9.24 and(3.64±0.36)%,respectively,all P<0.05].TEOA could increase ROS levels in DLBCL OCI-LY3 cells and effectively upregulate the expression levels of p38,p-Chk1,p-Chk2 andγ-H2AX in OCI-LY3.Conclusion TEOA can inhibit cell proliferation and invasion whilst effectively inducing cell apoptosis and ROS expression.TEOA acting on DLBCL OCI-LY3 cells can promote the expression of p38,p-Chk1,p-Chk2 andγ-H2AX and induce the activation of the p38 MAPK pathway.
作者
张贵兵
何正飞
商文忠
王爱伟
吴艳芳
黄惠燕
ZHANG Gui-bing;HE Zheng-fei;SHANG Wen-zhong;WANG Ai-wei;WU Yan-fang;HUANG Hui-yan(Department of Hematdogy,the First People's Hospital of Fuyang District,Hangzhou City,Hangzhou,Zhejiang 311400,China)
出处
《中华全科医学》
2022年第5期785-788,共4页
Chinese Journal of General Practice
基金
浙江省中医药科技计划项目(2020ZA098)。
关键词
弥漫大B细胞淋巴瘤
三乙醇胺
细胞
生物特性
Diffuse large B-cell lymphoma
Triethanolamine
Cell
Biological characteristics
